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V plex human ifnγ kit

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The V-PLEX Human IFNγ Kit is a multiplex immunoassay kit designed to measure the concentration of interferon-gamma (IFNγ) in human biological samples. The kit utilizes Mesoscale's electrochemiluminescence detection technology to provide a sensitive and quantitative measurement of IFNγ levels.

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Lab products found in correlation

Maxisorp immunoplate, by Thermo Fisher Scientific (1 mentions) Human tumor dissociation kit, by Miltenyi Biotec (1 mentions)

3 protocols using v plex human ifnγ kit

1

CD70 Costimulation Enhances T Cell Activation

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The costimulatory capability of CD70 protein on primary human T cells activation upon T cell receptor engagement was evaluated using plate-bound anti-CD3 antibody (Ultra-LEAF(TM) purified anti-human CD3 [OKT3], Biolegend, # 317326) as the TCR ligand. Anti-CD3 antibody and various concentration of CD70 protein in sterile PBS were added into MaxiSorp Immuno plates (Thermo Scientific, #439454) and incubated at 4 °C overnight. Next day the plates were washed three times with fresh PBS. Human Peripheral Blood pan-T cells (StemCell, # 70024) were added into the precoated plates and incubated at 37 °C, 5% CO2 for 3 days. The activation of T cells was evaluated by measuring the IFNγ production with V-PLEX Human IFN-γ Kit (Meso Scale Diagnostics, #K151QOD-4) following the manufacturer’s protocol. The data represent three independent biological replicates (mean± standard error).
Liu W., Maben Z., Wang C., Lindquist K.C., Li M., Rayannavar V., Lopez Armenta I., Nager A., Pascua E., Dominik P.K., Oyen D., Wang H., Roach R.C., Allan C.M., Mosyak L, & Chaparro-Riggers J. (2021). Structural delineation and phase-dependent activation of the costimulatory CD27:CD70 complex. The Journal of Biological Chemistry, 297(4), 101102.
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2

T Cell Activation by Dendritic Cells

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Primary human CD3+ pan T cells were freshly isolated from PBMCs from healthy donors using Dynabeads™ Untouched™ Human T Cells isolation kit (Thermo Fisher Scientific) and mixed with previously frozen monocyte-derived DCs from three different donors (HemaCare) at a 10:1 T to DC ratio. To this end, 105 T cells/well and 104 DCs/well were plated in 96-well round-bottom plates (Corning #4515). The cell mixture was then incubated with test antibody for 6 days. IFNγ levels in the supernatant were measured by V-PLEX Human IFNγ Kit (Meso Scale Discovery).
Geuijen C., Tacken P., Wang L.C., Klooster R., van Loo P.F., Zhou J., Mondal A., Liu Y.B., Kramer A., Condamine T., Volgina A., Hendriks L.J., van der Maaden H., Rovers E., Engels S., Fransen F., den Blanken-Smit R., Zondag-van der Zande V., Basmeleh A., Bartelink W., Kulkarni A., Marissen W., Huang C.Y., Hall L., Harvey S., Kim S., Martinez M., O’Brien S., Moon E., Albelda S., Kanellopoulou C., Stewart S., Nastri H., Bakker A.B., Scherle P., Logtenberg T., Hollis G., de Kruif J., Huber R., Mayes P.A, & Throsby M. (2021). A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade. Nature Communications, 12, 4445.
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3

Assaying Endometrial Tumor Immunity

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Surgically resected primary endometrial tumors (Avaden Biosciences) were dissociated into a single-cell suspension using a Human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer’s instructions and analyzed by flow cytometry for immune infiltration and surface marker expression. Cells were placed into 96-well round-bottom plates and incubated with 10 μg/ml test antibody in the presence of 0.01 μg/ml soluble anti-CD3 antibody (Clone Hit3a) for 6 days. IFNγ levels in the supernatant were measured by V-PLEX Human IFNγ Kit (Meso Scale Discovery).
Geuijen C., Tacken P., Wang L.C., Klooster R., van Loo P.F., Zhou J., Mondal A., Liu Y.B., Kramer A., Condamine T., Volgina A., Hendriks L.J., van der Maaden H., Rovers E., Engels S., Fransen F., den Blanken-Smit R., Zondag-van der Zande V., Basmeleh A., Bartelink W., Kulkarni A., Marissen W., Huang C.Y., Hall L., Harvey S., Kim S., Martinez M., O’Brien S., Moon E., Albelda S., Kanellopoulou C., Stewart S., Nastri H., Bakker A.B., Scherle P., Logtenberg T., Hollis G., de Kruif J., Huber R., Mayes P.A, & Throsby M. (2021). A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade. Nature Communications, 12, 4445.
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