Ip6k1

Manufactured by GeneTex

IP6K1 is a lab equipment product that functions as an inositol hexakisphosphate kinase enzyme. It catalyzes the phosphorylation of inositol hexakisphosphate (IP6) to form inositol pyrophosphates.

Automatically generated - may contain errors

Lab products found in correlation

Pgc 1α, by Santa Cruz Biotechnology (1 mentions) 3h acetic acid, by PerkinElmer (1 mentions) 9 10 3h n oleic acid, by PerkinElmer (1 mentions) Phospho ampk substrate, by Cell Signaling Technology (1 mentions) Pgc 1α, by Merck Group (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Acetylated lysine, by Cell Signaling Technology (1 mentions) 9 10 3h n palmitic acid, by PerkinElmer (1 mentions) Protein a g beads, by Merck Group (1 mentions) Retro x concentrator, by Takara Bio (1 mentions) Phsl s660, by Cell Signaling Technology (1 mentions) Rottlerin, by Bio-Techne (1 mentions) Gf109203x, by Bio-Techne (1 mentions) Cgi 58, by Santa Cruz Biotechnology (1 mentions) Plin1, by Cell Signaling Technology (1 mentions) Ly333531, by Bio-Techne (1 mentions) Cl316 243, by Merck Group (1 mentions) P pka, by Cell Signaling Technology (1 mentions) Insulin elisa kit, by Crystal Chem (1 mentions) Isoproterenol, by Merck Group (1 mentions)

Spelling variants of this product

Anti-IP6K1 (2 citations)

2 protocols using ip6k1

Quantifying Adipose Metabolism Regulators

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QPCR: TaqMan probes were purchased from Life Technologies (Grand Island, NY). Antibodies: UCP1 (cat#U6382): Sigma Aldrich, St. Louis, MO; phospho (S473; cat#4060) and total Akt (cat#2920), phospho (S79; cat#3661) and total ACC (cat#3676), phospho (T172; cat#2535) and total AMPK (cat#5831), phospho-AMPK Substrate (cat#5759), acetylated-Lysine (cat#9814): Cell Signaling Technology, Danvers MA; Adiponectin HMW/LMW (cat#5901): BioVision Milpitas, CA; IP6K1 (cat#GTX103949): GeneTex; PGC1α (for immunoblotting, cat#AB3242): EMD Millipore, Billerica, MA; PGC1α (for immunoprecipitation, cat#sc-13067), DIO2 (sc-98716): Santa Cruz Biotechnology, Dallas, TX. Radiochemicals: [9, 10-3H(N)]-oleic acid (cat#NET289001MC), [9, 10-3H(N)]-palmitic acid (cat#NET043001MC) and [3H] Acetic acid (cat#NET003005MC), PerkinElmer Inc., Boston, MA. Unless otherwise stated, all chemicals were purchased from Sigma Aldrich.

Zhu Q., Ghoshal S., Tyagi R, & Chakraborty A. (2016). Global IP6K1 deletion enhances temperature modulated energy expenditure which reduces carbohydrate and fat induced weight gain. Molecular Metabolism, 6(1), 73-85.

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Adipocyte Lipolysis Regulation Assay

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Antibodies: pPKA and pPKC substrate specific antibodies; PLIN1 and pHSL (S660): from Cell Signaling Technology. CGI-58, HSL, GST and β-Actin: from Santa Cruz Biotechnology. Myc: from Roche. IP6K1: from Genetex. Plasmid construct: pT7T3D-PacI-PLIN1 construct was purchased from GE Healthcare/Open Biosystems. Reagents and Kits: Glycerol, FFA and TAG assay kits: from Cayman Chemicals; insulin Elisa kit from Crystal Chem; BCA protein assay kit: Pierce Biotechnology; Jetprime transfection reagent from Polypus; RetroX concentrator: from Clontech; Cyclic AMP from Cell Signaling; protein A/G beads from EMD Millipore. Pharmacologic modulators: Forskolin/FSK (Cell Signaling), isoproterenol (Sigma), CL316243 (Sigma); Rottlerin, LY333531 and GF109203X (Tocris). Protease plus phosphatase inhibitor tablets; Thermo Scientific, Waltham, MA. Unless otherwise stated, all the chemicals are purchased from Sigma Aldrich.

Ghoshal S., Tyagi R., Zhu Q, & Chakraborty A. (2016). Inositol hexakisphosphate kinase-1 interacts with perilipin1 to modulate lipolysis. The international journal of biochemistry & cell biology, 78, 149-155.

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