Eugenol p.a, BF3-diethyl ether, methanol, anhydrous Na2SO4, AIBN (2,2’, Azobis(2-methylpropionitrile)), polysulfone and polyethylene glycol were purchased from Sigma-Aldrich (Jakarta, Indonesia). 0.1 M HCl, H2SO4, HNO3, chloroform p.a, 1000 ppm Au solution, NMP (1-Methyl-2-pyrrolidone), thiourea, and metals ions in the form of Cd(NO3)2, Cu(NO3)2, and Fe(NO3)2 were purchased from Merck (Jakarta, Indonesia) while aquabidest was purchased from Bratachem (Semarang, Indonesia).
0.1 m hcl
Manufactured by Merck Group
Sourced in Indonesia, Germany, United States
0.1 M HCl is a laboratory reagent solution of hydrochloric acid (HCl) with a molarity of 0.1 M. It is a clear, colorless liquid that is commonly used in various chemical and analytical applications in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Anhydrous na2so4, by Merck Group (1 mentions)
Polyethylene glycol, by Merck Group (1 mentions)
Thiourea, by Merck Group (1 mentions)
Chloroform p a, by Merck Group (1 mentions)
Cu no3 2, by Merck Group (1 mentions)
Cd no3 2, by Merck Group (1 mentions)
H2so4, by Merck Group (1 mentions)
Aa800 spectrometer, by Agilent Technologies (1 mentions)
Suprapur hno3, by Merck Group (1 mentions)
Nahco3, by Merck Group (1 mentions)
1 m hcl, by Carl Roth (1 mentions)
Orion star a211, by Thermo Fisher Scientific (1 mentions)
0.1 m naoh, by Merck Group (1 mentions)
5 protocols using 0.1 m hcl
1
Synthesis and Application of Polysulfone-Based Adsorbent
2
Quantification of Iron Content in s-SPIONs
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The iron content in the s-SPIONs was measured using an AAS instrument (Varian AA800 spectrometer, with acetylene/air flame atomization). An iron standard solution (1000 mg/L in HNO3, Merck) was used to prepare 2, 4, 6, 8, and 100 ppm standard solutions for the calibration curve. Samples of dried s-SPIONs were digested in Suprapur® HNO3 (500 µL for each sample, Merck-Millipore) at 70 °C, followed by dilution with 0.1 M HCl (Merck) to a final volume of 5.0 mL prior to measurement.
Either graphite furnace atomic absorption spectroscopy (GF-AAS, Agilent 240FS AA spectrometer with a Zeeman graphite tube atomizer; Agilent Technologies Australia, Mulgrave, VIC, Australia) or flame-AAS (Varian AA800 spectrometer, with acetylene/air flame atomization; Agilent Technologies Australia) were used to quantify iron content in the collected frozen tissues. For GF-AAS, a standard solution of iron in 1% (v/v) HNO3 at a concentration of 25 ppb was prepared from a commercial iron standard (Merck Millipore, 1000 mg/L in HNO3,) for a calibration curve at 5, 10, 15, and 20 ppb.
Either graphite furnace atomic absorption spectroscopy (GF-AAS, Agilent 240FS AA spectrometer with a Zeeman graphite tube atomizer; Agilent Technologies Australia, Mulgrave, VIC, Australia) or flame-AAS (Varian AA800 spectrometer, with acetylene/air flame atomization; Agilent Technologies Australia) were used to quantify iron content in the collected frozen tissues. For GF-AAS, a standard solution of iron in 1% (v/v) HNO3 at a concentration of 25 ppb was prepared from a commercial iron standard (Merck Millipore, 1000 mg/L in HNO3,) for a calibration curve at 5, 10, 15, and 20 ppb.
3
Simulated Gastroduodenal Digestion of Yoghurt
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The chemical digestion has been previously described by Rodríguez et al. 35 Procedures were performed as follows: 12.5 g of yoghurt was stirred in 50 mL of 0.1 M HCl (Merck) for 1 h at pH 1.0-2.0, at 30 rpm and 37 °C to reproduce the gastric environment. The pH was adjusted to 6.8-7.2 with 15 g per L of NaHCO 3 (Sigma Chemical Co., St Louis, MO, USA) and the stirring speed was increased from 30 to 300 rpm, while the temperature was maintained at 37 °C to reproduce the duodenal environment. All reagents were of analytical grade.
4
Synthesis of Polyaniline Nanostructures
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Polyaniline nanostructures were synthesized using interfacial polymerization according to the protocol reported by Abdolahi et al. [26 (link)]. Aniline (Fluka, Neu-Ulm, Germany) was distilled prior to use. Two solutions were prepared as follows: 20 mL of 1 M HCl (Carl Roth, Karlsruhe, Germany) aqueous solution with 4 mM of ammonium persulfate (Fluka, Neu-Ulm, Germany) and 20 mL of 4 mM of aniline solution in chloroform (Penta Praha, Czech Republic). Solutions were stirred separately for 1 h before synthesis. The aqueous solution was further slowly added above the organic phase. A reaction occurred for 24 h. After polymerization, the aqueous phase was collected and the green PANI precipitate was filtered and washed, first with 0.1 M HCl (Sigma-Aldrich, Steinheim, Germany) and then with i-propanol (Sigma-Aldrich, Steinheim, Germany), to remove impurities such as the oxidizing agent, oligomers, and the residual monomer. Synthesized PANI nanostructures were dried at room temperature.
5
Adjusting Media pH Levels
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To obtain media at specific pH levels (pH 6.0, pH 6.7, pH 7.4, pH 8.4, and pH 9.2), drops of 0.1 M HCl (Sigma, St. Louis, MO, USA) or 0.1 M NaOH (Sigma) were added gradually to the media until the desired pH level was reached. pH was monitored using a pH meter (ORION STAR A211, Thermo Scientific, Waltham, MA, USA).
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