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Colorimetric assay kit

Manufactured by Spinreact
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Sourced in Spain

The Colorimetric assay kit is a laboratory equipment designed to perform quantitative analysis of various analytes in a sample. It utilizes a colorimetric detection method, where the concentration of the analyte is determined by the intensity of the color developed in the reaction. The kit includes all the necessary reagents and materials required to conduct the analysis.

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Lab products found in correlation

Md41021, by Spinreact (1 mentions) Colorimetric assay kit, by Abcam (1 mentions) Elisa assay kit, by Cusabio (1 mentions)

Spelling variants of this product

Enzymatic colorimetric assay kits (2 citations)

3 protocols using colorimetric assay kit

1

Hepatic Lipid Extraction and Quantification

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Hepatic TG and cholesterol were extracted as described by Qu et al. [44] and determined using a colorimetric assay kit (Ref: 41 030 and MD41021, respectively) from Spinreact (Girona, Spain).
Velázquez A.M., Bentanachs R., Sala‐Vila A., Lázaro I., Rodríguez‐Morató J., Sánchez R.M., Alegret M., Roglans N, & Laguna J.C. (2022). ChREBP‐driven DNL and PNPLA3 Expression Induced by Liquid Fructose are Essential in the Production of Fatty Liver and Hypertriglyceridemia in a High‐Fat Diet‐Fed Rat Model. Molecular Nutrition & Food Research, 66(7), 2101115.
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2

Liver Function and Oxidative Stress Assays

Cited 1 time
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Liver function tests were carried out by kinetic measuring of serum alanine aminotransferase (ALT) activity [17 (link)] using a kit from BM Egypt (Cairo, Egypt) and a Beckman Coulter analyzer. Total protein (TP) content was determined in serum using a colorimetric assay kit (SPINREACT, Spain) [18 ]. Arginase activity was determined in liver tissue as described before [19 (link)] using a colorimetric assay kit (BioVision, California, USA). Superoxide dismutase (SOD) activity, malondialdehyde (MDA), and NO contents were determined in liver homogenate as described elsewhere [20 (link)–22 (link)], respectively. The level of inflammatory marker tumor necrosis factor-α (TNF-α) in serum was determined using an ELISA assay kit (CUSABIO, USA) [23 (link)]. Caspase-3 activity was detected by a colorimetric assay kit (MBL, Woburn, USA) [24 (link)].
Haroun A.M., El-Sayed W.M, & Hassan R.E. (2023). Quercetin and l-Arginine Ameliorated the Deleterious Effects of Copper Oxide Nanoparticles on the Liver of Mice Through Anti-inflammatory and Anti-apoptotic Pathways. Biological Trace Element Research, 202(7), 3128-3140.
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3

Hepatic Lipid Extraction and Triglyceride Quantification

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Lipids were extracted from hepatic tissue (1 g) utilizing the method of Folch et al. 19 . Total lipids were extracted from liver samples by homogenizing the tissues using a mixture of chloroform/methanol/0.9% NaCl to a final 20fold dilution of the original volume of the tissue sample. The organic layer was then removed, evaporated, and reconstituted in chloroform. The values of TG were measured using a colorimetric assay kit (Spinreact, Barcelona, Spain) and the results were expressed as mg per gram of the liver weight.
Castro R.Q., Soto Rodriguez I., Deschamps Lago R.A., Pagola P.G., Rodriguez Antolin J., Peres Quintal A., Rivera J.R, & Aguilera A.A. (2018). Dietary sucrose regulates the expression of the Cd36 gene in hepatic tissue of rats with obesity and Non Alcoholic Fatty Liver Disease (NAFLD). Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia, 162(2).
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