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Fitc conjugated mouse anti chicken cd8α antibody

Manufactured by Southern Biotech
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Sourced in United States

The FITC-conjugated mouse anti-chicken CD8α antibody is a laboratory reagent used for the identification and characterization of CD8α-positive cells in chicken samples. It is a fluorescently labeled monoclonal antibody that binds specifically to the CD8α cell surface protein, which is expressed on a subset of T lymphocytes. This product can be used for various applications, such as flow cytometry, immunohistochemistry, and cell sorting, to detect and analyze CD8α-positive cells in biological samples.

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Lab products found in correlation

Pe conjugated mouse anti chicken cd3 antibody, by Southern Biotech (2 mentions) Fitc conjugated mouse anti chicken cd4 antibody, by Southern Biotech (2 mentions) Facsc 2000, by BD (2 mentions)

Spelling variants of this product

Mouse anti-chicken CD8α-FITC (3 citations) CD8α-FITC (3 citations)

2 protocols using fitc conjugated mouse anti chicken cd8α antibody

1

Chicken Antibody and T-cell Response Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 0 and 5 dpi, the serum antibodies of chickens were detected by enzyme-linked immunosorbent assay (ELISA) as described previously (56 (link)). All samples were analyzed in triplicate.
To measure the percentages of CD4+ and CD8+ cells among CD3+ cells, peripheral blood lymphocytes (PBLs) were incubated with phycoerythrin (PE)-conjugated mouse anti-chicken CD3 antibody (Southern Biotech, USA) and fluorescein isothiocyanate (FITC)-conjugated mouse anti-chicken CD4 antibody (Southern Biotech) or FITC-conjugated mouse anti-chicken CD8α antibody (Southern Biotech) at 4°C for 1 h. After being washed with PBS, the PBLs were analyzed by fluorescence-activated cell sorter (FACS; FACSC 2000, BD).
Zhao N., Ming S., Sun L., Wang B., Li H., Zhang X, & Zhao X. (2021). Identification and Characterization of Eimeria tenella Microneme Protein (EtMIC8). Microbiology Spectrum, 9(1), 10.1128/spectrum.00228-21.
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2

Chicken Antibody and T-cell Response Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 0 and 5 dpi, the serum antibodies of chickens were detected by enzyme-linked immunosorbent assay (ELISA) as described previously (56 (link)). All samples were analyzed in triplicate.
To measure the percentages of CD4+ and CD8+ cells among CD3+ cells, peripheral blood lymphocytes (PBLs) were incubated with phycoerythrin (PE)-conjugated mouse anti-chicken CD3 antibody (Southern Biotech, USA) and fluorescein isothiocyanate (FITC)-conjugated mouse anti-chicken CD4 antibody (Southern Biotech) or FITC-conjugated mouse anti-chicken CD8α antibody (Southern Biotech) at 4°C for 1 h. After being washed with PBS, the PBLs were analyzed by fluorescence-activated cell sorter (FACS; FACSC 2000, BD).
Zhao N., Ming S., Sun L., Wang B., Li H., Zhang X, & Zhao X. (2021). Identification and Characterization of Eimeria tenella Microneme Protein (EtMIC8). Microbiology Spectrum, 9(1), 10.1128/spectrum.00228-21.
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