Dual pam 100 chl

Manufactured by Walz

Sourced in Germany

The Dual-PAM 100 Chl is a laboratory instrument designed for the assessment of chlorophyll fluorescence. It provides simultaneous measurement of chlorophyll fluorescence parameters from two separate samples or sample areas.

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Whatman, by Cytiva (1 mentions)

Spelling variants of this product

Dual-PAM-100 (172 citations) Dual-PAM (16 citations) Dual-PAM-100 Chl fluorescence photosynthesis analyzer (2 citations)

2 protocols using dual pam 100 chl

Photosynthesis and Chlorophyll Fluorescence

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The net photosynthetic rate (Pn) of the second true leaf was measured using a Li-6400 portable photosynthesis system (Licor-6400, LICOR Inc., Lincoln NE, USA) with a red/blue light source according to the manufacturer’s instructions. The photosynthetic photon flux density (PPFD) and the temperature and external CO₂ concentration was set at 1000 μmol m^− 2 s^− 1, 30 °C and 400 μmol mol^− 1, respectively.
The maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm) was measured with a Dual-PAM 100 Chl fluorescence analyzer (Heinz Walz, Effeltrich, Germany) after a 30 min dark-adaptation following the method of Wang et al. [51 ]. The Fv/Fm images were detected by Chlorophyll fluorescence Imager (CF Imager) (Technologica, United Kingdom, http://www.technologica. co.uk/).

Zhan Y., Wu T., Zhao X., Wang Z, & Chen Y. (2021). Comparative physiological and full-length transcriptome analyses reveal the molecular mechanism of melatonin-mediated salt tolerance in okra (Abelmoschus esculentus L.). BMC Plant Biology, 21, 180.

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Functional Antenna Size of PSI

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The functional antenna size of PSI was estimated by the rate of light induced oxidation of P 700 RC under light limiting conditions. The oxidation kinetics of P 700 upon illumination was followed by the measurement of absorbance change at 830 nm using Dual-PAM 100 Chl a fluorometer (Walz, Germany). Prior to measurement samples were darkadapted for 3 min in the presence of 100 mM MV and 20 mM DCMU then cell suspension equivalent to 20 mg Chl was filtered onto a 25-mm diameter glass fiber syringe filter disc (Whatman GF/C). The filter discs, placed between two microscopy slides with a spacer, were inserted between the fiber optics of the emitter-detector unit. Samples were illuminated with 5-s long 635-nm pulses at various intensities (6, 31, 140, 251, and 805 mmol photon m -2 s -1 ) consecutively and the oxidation kinetics were recorded at a millisecond sampling rate.

Akhtar P., Biswas A., Balog-Vig F., Domonkos I., Kovács L, & Lambrev P.H. (2022). Trimeric photosystem I facilitates energy transfer from phycobilisomes in Synechocystis sp. PCC 6803. Plant physiology, 189(2).

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