Hit8a

Manufactured by BD

The HIT8a is a laboratory equipment product. It is designed to perform core functions within a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Ly6g 1a8, by BD (1 mentions) Hit3a, by BD (1 mentions) Rpa t4, by BD (1 mentions) Ifn γ xmg1.2, by BD (1 mentions) Lsrfortessa x 20 flow cytometer, by BD (1 mentions) Mq1 17h12, by Thermo Fisher Scientific (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Facsymphony, by BD (1 mentions)

Close spelling variants of this product

Mouse anti-human CD8 (clone HIT8a, Clone HIT8A αCD8-APC (clone HIT8a; CD8-PE (clone HIT8a, CD8-FITC (clone HIT8a, Anti-CD8-PE (clone HIT8a, CD8 HIT8a CD8 (clone HIT8a),

2 protocols using hit8a

Multiparametric Flow Cytometry of Immune Cells

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Antibodies against human CD3 (HIT3a), CD4 (RPA-T4), CD8 (HIT8a), CD54 (HA58), TNF-α (7N4–1) and INF-γ (B27) were from BD Biosciences (San Jose, CA). Antibodies against human CD106 (STA) and CD62E (P2H3) were from Thermo Fisher Scientific (Canoga Park, CA). Antibodies against mouse CD3 (145–2C11), CD4 (RM4–5), CD8 (53–6.7), CD54 (3E2), CD62E (10E9.6), CD11c (HL3), CD11b (M1/70), CD40 (HM40–3), CD11c (HL3), CD11b (M1/70), CD45.2 (104), CD31 (MEC 13.3), and Ly-6G (1A8), and IFN-γ (XMG1.2) were from BD Biosciences. Anti-mouse CD106 (429) was from Thermo Fisher Scientific. Intracellular IFN-γ and TNF staining was performed as described previously [31 (link)]. Flow cytometric data was acquired using an LSRFortessa™ X-20 flow cytometer (BD Biosciences), and events were analyzed using FlowJo software (Version 9.9.6, TreeStar, San Carlos, CA).

Lin L., Xu H., Bishawi M., Feng F., Samy K., Truskey G., Barbas A.S., Kirk A.D, & Brennan T.V. (2019). Circulating mitochondria in organ donors promote allograft rejection. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19(7), 1917-1929.

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Immunophenotyping by Flow Cytometry

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The following antibodies were used for flow cytometry analysis: CD8 (BD Biosciences, HIT8a), CD4 (BD Biosciences, SK3), IFNg (Invitrogen, 4S.B3), CD3 (eBioscience, SK7), TNFα (eBioscience, MAb11), IL-2 (Invitrogen, MQ1-17H12). The following antibodies were purchased from BioLegend: CD25 (BC96), CD3 (SK7), TNFα (MAb11), CD8 (RPA-T8), CD4 (OKT4). Stimulatory anti-CD3 (OKT3) and anti-CD28 (CD28.2) antibodies were from BioLegend. Flow cytometry was performed on a FACSymphony or LSR II (BD Biosciences). Data from flow cytometry was analyzed via FlowJo software (BD Biosciences).

Burton A., Ligman B.R., Kearney C.A., & Murray S. (2021). Clinically relevant SMAC mimetics do not enhance human T cell proliferation or cytokine production.

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