Antibodies against sirt1

Oleic acid, linOleic acid, palmitic acid, stearic acid and palmitOleic acid were obtained from Sigma (St. Louis, MO, USA). The mimics, inhibitors and Ago-181a or Ago-NC were purchased from RiboBio (RiboBio Co. Ltd, Guangzhou, China). X-tremeGENE siRNA Transfection Reagent was from Roche (Mannheim, Germany). The mammalian cell protein extraction kit was from Beyotime (Jiangsu, China). The antibodies against acety-lysine (Cat: 9441), tyr1150/1151-phosphorylated IR (Cat: 3024; 95 kDa), Akt (Cat: 9272; 60 kDa), Ser473-phosphorylated Akt (Cat: 4060; 60 kDa) and Ser9-phosphorylated (GSK3β) (Cat: 9336; 46 kDa) were purchased from Cell Signaling Technology(Berverly, MA, USA), antibody against SREBP-1c (Cat: NB100-2215; 65 kDa) (Novus Biologicals, Littleton, CO, USA), antibodies against β-actin (Cat: ab8226; 42 kDa), PPARα (Cat: ab8934; 52 kDa), IR (Cat: ab69508; 95kDa) and GSK3β (Cat: ab69739; 47 kDa) were purchased from Abcam (Cambridge, England), antibodies against SIRT1 (Cat: sc-15404; 120 kDa) and PGC-1α (Cat: sc13067; 90 kDa) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Bovine SIRT1 small interference RNA (siRNA) or control, adenovirus expressing bovine SIRT1 (Ad-SIRT1), Ad-shRNA SIRT1 or negative control shRNA (Ad-shRNA NC) were purchased from Hanbio (Shanghai, China)

Antibodies against SIRT1, CK2α, p53-p21^Cip1/WAF1, RelA/p65, IκB, α-tubulin, and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against acetylated RelA/p65 (ac-p65, K310), IKKα/β, phosphorylated IKKα/β (p-IKKα/β, S180/S181), IL-1β, IL-6, MMP3, and histone H3 were obtained from Abcam (Cambridge, UK). Antibodies against phosphorylated IκBα (p-IκBα, S32/S36) and phosphorylated AKT (p-AKT, S473) were obtained from Cell Signaling (Danvers, MA, USA). Resveratrol, triciribine, nicotine amide, and LPS were obtained from the Sigma Chemical Co. (St. Louis, MO, USA).