Beyoclick edu kit with alexa fluor 488

Cell proliferation was measured using a BeyoClick™ EDU kit with Alexa Fluor 488 (#C0071S, Beyotime, Shanghai, China). BEAS-2B cells were seeded into a six-well plate overnight. After being stimulated by LPS at a concentration of 10 µg/mL for 24 h, the cells were cultured in EDU dyeing solution for 2 h at a final concentration of 10 µM. After using the BeyoClick EDU Cell Proliferation Kit with Alexa Fluor 488, Hoechst 33342 was utilized for nuclear staining according to the manufacturer's instructions. Images were captured using a microscope (Olympus IX51, Tokyo, Japan).

The cell proliferation was analyzed using BeyoClick™ EdU Kit with Alexa Fluor 488 (Beyotime, China). Twenty-four hours after transfection, U251 and U87 cells (1 × 10⁴ cells per well) were seeded in a 96-well plate for 24 h and then exposed to 50 µM EdU for 2 h at 37 °C. After fixed with 4% formaldehyde for 15 min, the cells were infiltrated with 0.3% Triton X-100 for 15 min. After washing, the cells were treated with 100 µl Click Additive Solution for 30 min and counterstained using Hoechst33342. The staining results were calculated under fluorescence microscope (IX-71, Olympus, Tokyo, Japan). Pictures of five fields of view randomly selected were taken to further analyze.