Glyceryl trimyristate

The following lipids were obtained from Sigma-Aldrich (Buchs, Switzerland): oleamide, stearamide, glyceryl tristearate, glyceryl trimyristate, glyceryl tripalmitate, palmitoleic acid, oleic acid, and cholesterol. The following lipids were obtained from Avanti Polar Lipids (Alabaster, AL, USA): PC(16:0/20:4), PC(17:0/17:0), PC(18:0/18:0), PC(18:2/18:2), PC(20:4/20:4), PC(22:6/22:6), PC(19:0/19:0), PC(16:0/16:0), LPC(16:0), LPC(17:0), LPC(18:1), LPC(18:0), LPA(17:0), LPA(18:0), LPA(20:4), LPA(18:1), LPA(14:0) and LPA(16:0). Standards were dissolved in propan-2-ol in groups of 10 to 20 in the concentration range 10 to 80 μM for analysis by UPLC-ESI-QTOFMS (see below). oleamide was analyzed by GC-MS (see below).

Cisplatin, stearic acid, Tween 80 and glyceryl trimyristate were purchased from Sigma-Aldrich, Durban, South Africa. MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and solvents used in the studies were either bought from Merck or Sigma-Aldrich, Durban, South Africa, and were of analytical grade. The pH study was performed with 0.1 M phosphate buffer which included sodium dihydrogen orthophosphate dehydrates (Merck, South Africa) and sodium dibasic dehydrates (Sigma-Aldrich, Taufkirchen, Germany). Throughout the experiment, double distilled water (DDW) was used.