Anti cox4 11242 1 ap

Western blot analyses were performed conventionally using the following rabbit antibodies: anti-SLIRP (26006-1-AP, Proteintech, Manchester, UK), anti-MRPS27 (17280-1-AP, Proteintech, Manchester, UK), anti-MRPL44 (16394-1-AP, Proteintech, Manchester, UK), anti-Cox4 (11242-1-AP, Proteintech, Manchester, UK), anti-COII (55070-1-AP, Proteintech, Manchester, UK), anti-COIII (55082-1-AP, Proteintech, Manchester, UK), anti-CytB (55090-1-AP, Proteintech, Manchester, UK), anti-NDUFS3 (15066-1-AP, Proteintech, Manchester, UK), anti-Atp5A1 (14676-1-AP, Proteintech, Manchester, UK), and ECL Anti-rabbit IgG HRP linked (NA934V, GE Healthcare, MA, USA), according to the manufacturer’s protocols. Blots were stained with SuperSignal West Pico ECL reagents (ThermoFisher Scientific, Waltham, MA, USA) and visualized with ChemiDoc imaging system (Bio-Rad, CA, USA).

The chemicals used in our experiments were CCCP (HY-100941), liensinine (HY-N0484), valinomycin (HY-N6693), and cisplatin (HY-17394), which were purchased from MedChemExpress. Doxorubicin (S1208) were purchased from Selleckchem. Pemetrexed (SML1490) was purchased from Sigma-Aldrich.
The primary antibodies used in the western blot and immunoprecipitation assays were as follows: anti-ACTB (A1978), anti-GFP (G1544), anti-FLAG (F7425, F1804), anti-GAPDH (G8795), and anti-MYC (C3956, M4439) were obtained from Sigma-Aldrich; anti-LC3B (2775), anti-ATG5 (9980), anti-acetylated lysine (6952),anti-CASP8 (9746), anti-Parkin (4211), ATG7 (8558) were obtained from Cell Signaling Technology; anti-TIMM23 (11123-1-AP), anti-TOMM20 (11802-1-AP), anti- MFN1 (66776-1-Ig), and anti-COX4 (11242-1-AP) were obtained from Proteintech; anti-BEX2 (SC-398486), anti-HSP60 (SC-13115), and anti-NDP52 (SC-376540) were obtained from Santa Cruz Biotechnology; anti-CASP3 (NB100-56708) was obtained from Novus Biologicals; anti-PARP-1 (556494) was obtained from BD Biosciences; and anti-pan-Kcr (PTM-502) was obtained from PTM Biolabs.

Quantitative real-time PCR was performed after reverse transcription with PrimeScript^TM RT reagent kit (RR047A, Takara, Mountain View, CA, United States) with gene-specific primers and SYBR Green PCR Master Mix (43-676-59, Applied Biosystems, Foster City, CA, United States). Western immunoblotting was performed as described previously (Li et al., 2018 (link)). Anti-BNIP3 (3769, CST), anti-PLIN2 (15294-1-AP, Proteintech, Rosemont, IL, United States), anti-COX4 (11242-1-AP, Proteintech), and anti-GAPDH (HC301-01, Transgen, Strasbourg, France) were used. siRNA transfection was performed with Lipofectamine RNAiMAX (13778-075, Thermo Fisher) according to the manufacturer’s recommended protocol. Sequences of primers and siRNAs are provided in Supplementary Table 1.