Eclipse te2000 e inverted microscope

For live imaging, embryos were collected for 15 min to 1 hr, depending on the stage to be imaged, dechorionated with 4.2% sodium hypochlorite for 2 minutes, washed with dH₂O, mounted as a monolayer of embryos in a drop of halocarbon oil (Series 56) on the outer surface of the Greiner Lumox culture dishes (Sigma-Aldrich Cat# Z376744), and covered with a coverslip before imaging. All movies were captured using a 40x Planapochromat objective (NA 1.5) on a Nikon Eclipse TE2000-E inverted microscope with a Perkin-Elmer spinning disk and a Hamamatsu Orca-EM CCD camera. Movies were processed using the Metamorph software package (Universal Imaging, Downington, Pennsylvania).

For live imaging, embryos were collected for 15 min to 1 hour, depending on the stage to be imaged, dechorionated with 4.2% sodium hypochlorite for 2 min, washed with dH₂O, mounted as a monolayer of embryos in a drop of halocarbon oil (series 56) on the outer surface of the Greiner Lumox culture dishes (Sigma-Aldrich, catalog no. Z376744), and covered with a coverslip before imaging. All movies were captured using a 40× Planapochromat objective [numerical aperture (NA) 1.5] on a Nikon Eclipse TE2000-E inverted microscope with a Perkin-Elmer spinning disk and a Hamamatsu Orca-EM CCD camera. Movies were processed using the Metamorph software package (Universal Imaging, Downington, Pennsylvania).