Ab233615

Blood was collected via intracardiac puncture and immediately mixed with ethylenediaminetetraacetic acid (EDTA). The EDTA-blood solution was centrifuged for 15 min at 3000g, as previously described [9] (link). Plasma was collected and snap-frozen in liquid nitrogen. Levels of proteins of interest were measured using colorimetric enzyme-linked immunosorbent assays species-specific following the manufacturer's instruction. Specifically, DY3178 was used for TF (R&D systems, Minneapolis, MN, USA), ab233615 was used for tissue-plasminogen activators (tPA; Abcam, Cambridge, UK), DY3828 was used for plasminogen activator inhibitor 1 (R&D systems, Minneapolis, MN, USA), abx258705 was used for D-dimer (Abbexa, Cambridge, UK), MPS00 was used for soluble P-selectin (R&D systems, Minneapolis, MN, USA), NBP2-68171 was used for von Willebrand factor (vWF; Novus Biologicals, Cambridge, UK), NBP2-60632 was used for antithrombin III (Novus Biologicals, Cambridge, UK), and ab137994 was used for thrombin-antithrombin complexes (Abcam, Cambridge, UK).