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Alexa fluor647 anti rabbit antibodies

Manufactured by Thermo Fisher Scientific
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Alexa Fluor647 anti-rabbit antibodies are fluorescently-labeled secondary antibodies that bind to rabbit primary antibodies. They are used to detect and visualize target proteins or antigens recognized by rabbit primary antibodies in various biological applications.

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Lab products found in correlation

Lsm 880, by Zeiss (4 mentions) Ab27671, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Cell Signaling Technology (2 mentions) Hpa021799, by Merck Group (2 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (2 mentions)

2 protocols using alexa fluor647 anti rabbit antibodies

1

Immunofluorescence of V5 and GOLGA2

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded on cover slips and fixed with 4% paraformaldehyde in PBS for 10 minutes at room temperature. Cells were permeabilized with 1% NP-40 in antibody dilution solution (PBS, 0.2% BSA, 0.02% sodium azide) for 10 minutes and blocked with 1% goat serum for 45 minutes. Cells were incubated with anti-V5 (1:250, Abcam ab27671) and anti-GOLGA2 antibodies (1:250, Sigma HPA021799) for 1 hour at room temperature. Subsequently, cells were incubated with Alexa Fluor488 goat anti-mouse (1:500, Invitrogen A-11001) or Alexa Fluor647 anti-rabbit antibodies (1:500, Invitrogen A-21245) and counterstained with 1 μg/ml DAPI (Cell Signaling Technology, 4083S) for 45 minutes in the dark. Cells were visualized by confocal microscopy (Zeiss LSM 880).
Aregger M., Lawson K.A., Billmann M., Costanzo M., Tong A.H., Chan K., Rahman M., Brown K.R., Ross C., Usaj M., Nedyalkova L., Sizova O., Habsid A., Pawling J., Lin Z.Y., Abdouni H., Wong C.J., Weiss A., Mero P., Dennis J.W., Gingras A.C., Myers C.L., Andrews B.J., Boone C, & Moffat J. (2020). Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism. Nature metabolism, 2(6), 499-513.
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2

Immunofluorescence of V5 and GOLGA2

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded on cover slips and fixed with 4% paraformaldehyde in PBS for 10 minutes at room temperature. Cells were permeabilized with 1% NP-40 in antibody dilution solution (PBS, 0.2% BSA, 0.02% sodium azide) for 10 minutes and blocked with 1% goat serum for 45 minutes. Cells were incubated with anti-V5 (1:250, Abcam ab27671) and anti-GOLGA2 antibodies (1:250, Sigma HPA021799) for 1 hour at room temperature. Subsequently, cells were incubated with Alexa Fluor488 goat anti-mouse (1:500, Invitrogen A-11001) or Alexa Fluor647 anti-rabbit antibodies (1:500, Invitrogen A-21245) and counterstained with 1 μg/ml DAPI (Cell Signaling Technology, 4083S) for 45 minutes in the dark. Cells were visualized by confocal microscopy (Zeiss LSM 880).
Aregger M., Lawson K.A., Billmann M., Costanzo M., Tong A.H., Chan K., Rahman M., Brown K.R., Ross C., Usaj M., Nedyalkova L., Sizova O., Habsid A., Pawling J., Lin Z.Y., Abdouni H., Wong C.J., Weiss A., Mero P., Dennis J.W., Gingras A.C., Myers C.L., Andrews B.J., Boone C, & Moffat J. (2020). Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism. Nature metabolism, 2(6), 499-513.
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