N-Ac-PGP was provided by Sigma-Aldrich (St. Louis, MO, USA). N-Ac-PGP was diluted with phosphate-buffered saline (PBS; 1 mg/ml). The rabbit monoclonal anti-human p38 (no. 9212), phos pho-p38 (p-p38; no. 9211), JNK (no. 9252), p-JNK (no. 9251), ERK (no. 9102) and p-ERK (no. 9101) antibodies were all obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). The mouse monoclonal anti-human GAPDH (sc-47724) antibody was provided by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The rabbit polyclonal anti-human p65 antibody (ab16502) and the rabbit monoclonal anti-human p-p65 antibody (ab76302) were both purchased from Abcam (Cambridge, MA, USA). The goat polyclonal anti-mouse IgG (H+L) horseradish peroxidase (HRP)-conjugated seco ndary antibody (ZB2305) and the goat anti-rabbit IgG (H+L) HRP-conjugated secondary antibody (ZB2301) were both purchased from ZSGB-BIO (Beijing, China). The p38 (SB202190, SB), JNK (SP600125, SP) and ERK (U0126, U) signaling inhibitors were provided by MedChem Express (Princeton, NJ, USA). The NF-κB inhibitor (PDTC) was purchased from Beyotime Institute of Biotechnology (Shanghai, China).
Goat anti rabbit igg h l hrp conjugated secondary antibody zb2301
Manufactured by ZSGB-BIO
Sourced in China
Goat anti-rabbit IgG (H+L) HRP-conjugated secondary antibody (ZB2301) is a laboratory reagent used to detect and visualize rabbit primary antibodies in various immunoassays. It is composed of goat-derived antibodies that are specific to the heavy and light chains of rabbit immunoglobulin G (IgG), and are conjugated to the enzyme horseradish peroxidase (HRP).
Automatically generated - may contain errors
2 protocols using goat anti rabbit igg h l hrp conjugated secondary antibody zb2301
1
Investigating MAPK and NF-κB Signaling
2
Immunoblotting Antibody Characterization
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The mouse monoclonal anti-rat glyceraldehyde-3-phosphate dehydrogenase (GAPDH, sc-47724), p53 (sc-126), p21 (sc-6246), p16 (sc-1661) and the rabbit polyclonal anti-rat retinoblastoma protein (Rb, sc-50) antibody were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The rabbit monoclonal rabbit anti-rat phospho-histone γ-H2A.X (Ser139) antibody was obtained from Cell Signaling Technology (#9718; Danvers, MA, USA). The donkey polyclonal anti-mouse IgG Alexa Fluor® 647-conjugated secondary antibody (AP192SA6) and the goat polyclonal goat anti-rabbit IgG (H+L) Alexa Fluor® 647-conjugated secondary antibody (AP187SA6) were purchased from Merck Millipore (Billerica, MA, USA). The goat polyclonal anti-mouse IgG (H+L) horseradish peroxidase (HRP)-conjugated secondary antibody (ZB2305) and the goat anti-rabbit IgG (H+L) HRP-conjugated secondary antibody (ZB2301) were purchased from ZSGB-BIO (Beijing, China).
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