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5 protocols using rebamipide

1

Synthesis and Characterization of Aldehyde Compounds

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Dansyl hydrazine (DH), glyoxal, crotonaldehyde, 2,4-nonadienal (NDE), 2,4-decadienal (DDE), heptadecanal, hexadecanal, irsogladine maleate, octadecanal, pentadecanal, rebamipide, teprenone, tetradecanal and troxipide were purchased from Tokyo Chemical Industry (Tokyo, Japan). The NF-κB inhibitor SM-7368 was obtained from Merck KGaA (Darmstadt, Germany). p-Toluenesulfonic acid (p-TsOH) and the RCs including propanal, pentanal, butanal, 2-hexenal, hexanal, 2-heptenal, heptanal, octanal, 2-nonenal, nonanal, decanal, undecanal, dodecanal and tridecanal were obtained from Sigma-Aldrich (St. Louis, MO, USA). 4-Hydroxy-2-hexenal (HHE), 4-hydroxy-2-nonenal (HNE), 4-oxo-2-nonenal (ONE) and 4,5-epoxy-2-decenal (EDE) were purchased from the Cayman Chemical Company (Ann Arbor, MI, USA). Ecabet sodium hydrate, p-benzyloxybenzaldehyde (p-BOBA), sofalcone and other chemicals used in the current study were purchased from Wako Pure Chemical Industries (Osaka, Japan). 8-Heptadecenal (8-HpDE), 8,11-heptadecadienal (8,11-HpDDE) and 8,11,14-heptadecatrienal (8,11,14-HpDTE) were synthesized by a method previously described [26 (link), 27 (link)]. Secosterol-A and -B were synthesized according to the procedure reported by Wentworth et al. [28 (link)]. Stock solutions of the RCs and an internal standard (IS) (p-BOBA, 10 mM) were prepared separately in acetonitrile and stored at −20°C prior to use.
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2

Methylcellulose Foams: Formulation and Characterization

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Materials Three viscosity grades of methylcellulose (SM-4, -100, and -400, Shin-Etsu Chemical Co., Ltd., Tokyo, Japan) and a foam pump (Pump Foamer, F7, estimate discharge volume, 0.8 g; Daiwa Can Company, Tokyo, Japan) were kindly provided for use in this study. Benzydamine (Comb-Blocks, Inc., San Diego, CA, U.S.A.), dexamethasone (Comb-Blocks, Inc.), allopurinol and rebamipide (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) were purchased. All reagents used for the study were reagent-grade products.
Preparation of Foam We prepared 12 different foams from 3 to 5 different concentrations of the three types (viscosity grades) of methylcellulose. Methylcellulose (SM-4, -100, and -400) were added in purified water with stirring, and prepared to 1, 2, 4, 6 and 8% for SM-4, 0.2, 0.6 and 1% for SM-100, and 0.1, 0.4, 0.6 and 0.8% for SM-400. Each methylcellulose solution was added to a foam pump, and each foam was generated by discharging from the pump. The foams were used for all tests after the priming of pumping (discharging the foam for 6 times).
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3

Rebamipide-Loaded PLGA Nanoparticles for Chondrocyte Therapy

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The mPEG-PDLLA (molecular weight [MW]: 5,000–20,000 Da) was purchased from Akina Inc. (West Lafayette, IN, USA). PLGA (50:50; Resomer® RG505) was supplied by Boehringer Ingelheim (Ingelheim, Germany). Rebamipide was purchased from the Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Dimethyl sulfoxide (DMSO) and lipopolysaccharide (LPS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). A cellulose-ester dialysis membrane (MW cut-off [MWCO]: 6–8 kDa) was obtained from Spectrum Laboratories (CA, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, phosphate-buffered saline (PBS), IL-1β and penicillin-streptomycin were obtained from Gibco BRL (Rockville, MD, USA). Chondrocytes were purchased from Lonza Ltd. (Walkersville, MD, USA).
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4

Comprehensive Pharmacological Inventory Protocol

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Betamethasone, amlodipine, insulin, lansoprazole, loxoprofen, metformin and warfarin were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan); allopurinol, famotidine, magnesium oxide and D-pantothenic acid were obtained from Nacalai Tesque, Inc. (Kyoto, Japan); aspirin was obtained from Merck Hoei Ltd. (Osaka, Japan); candesartan was purchased from Toronto Research Chemicals Inc. (North York, ON, Canada); and rebamipide was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All other chemicals used were of analytical grade.
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5

Screening of Small Molecule Compounds

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Esmolol hydrochloride, dequalinium dichloride, fosinopril, antimycin A, and oxprenolol hydrochloride were purchased from Santa Cruz Biotechnology. Alexidine dihydrochloride, merbromin, candesartan, amphotericin B, alprenolol hydrochloride, triprolidine hydrochloride, methacycline hydrochloride, cefixime, and ethacrynic acid were purchased from Sigma-Aldrich. Demecarium bromide and cefoperazone were purchased from AK Scientific. Bisoprolol fumarate was purchased from MedChem Express. Benzbromarone, etifenin, cefotetan, and rebamipide were purchased from Tokyo Chemical Industry Co., Ltd. Atractyloside potassium salt was purchased from Toronto Research Chemicals. Furosemide and indomethacin were purchased from Nacalai Tesque. Acemetacin, ketoprofen, bumetanide, colistin sulfate, tranilast, norfloxacin, and doxepin hydrochloride were purchased from LKT Laboratories, Inc.
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