Cd56 apc cy7

First, 100 μl of bone marrow (BM) samples were collected from MDS patients or HCs. Then, the bone marrow aspirations were stained with the following antibodies (BD Biosciences) at 4 °C for 15 min in the dark. Then 2 ml of lysing solution (BD Biosciences) was added to each tube to lyse erythrocytes for 10 min in the dark at room temperature, centrifuged at 1500 rpm for 5 min, and washed twice with phosphate-buffered saline (PBS). Finally, we analyzed the cells by FCM after washing the cells twice with phosphate-buffered saline (PBS). CytExpert Software (Beckman CytoFLEX) was used to analyze the FCM data.
The following antibodies (BD Biosciences) were used in this study: CD3-PerCP, CD56-APC-Cy7, TIGIT-FITC, CD226-APC, CD96-PE, NKG2D-PE-Cy7, CD107a-Bv421, IFN-γ-PE-Cy7, Perforin-Bv421, CD226-FITC, TIM-3-APC-Cy7, CD96-PE-Cy7, VISTA-PE-Cy7, PD-1- Bv421, LAG-3-Bv421, CD4-APC, CD8-PE, and CD56-PE. NK cells were labeled with CD3⁻CD56⁺, CD4⁺T cells with CD3⁺CD4⁺, and CD8⁺T cells with CD3⁺CD8⁺.