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Fixation buffer

Manufactured by Cytek Biosciences
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Fixation buffer is a laboratory reagent used to preserve the structural integrity of cells or tissues. It functions by chemically fixing and stabilizing cellular components, preventing degradation or morphological changes. The buffer composition and pH are designed to maintain the sample in a fixed state suitable for downstream analysis or processing.

Automatically generated - may contain errors

Lab products found in correlation

Cd16 32, by Cytek Biosciences (2 mentions) Ghost viability dye bv540, by Cytek Biosciences (2 mentions)

2 protocols using fixation buffer

1

Multiparameter Flow Cytometry Staining

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Cells were stained in the presence of 1:500 Fc block (CD16/32, Tonbo) and antibodies diluted 1:200 in FACs buffer (PBS+2.5% FBS) for 45 minutes in the dark at 4°C. Ghost viability dye BV540 (Tonbo) was used to exclude dead cells at 1:500. Cells were fixed in 2% PFA or fixation buffer (Tonbo) for 10–15 at room temperature in the dark prior to data acquisition. Cells were acquired within 24 h using a Cytek Aurora.
Ben-Artzi H., Zeelon E., Gorecki M, & Panet A. (1992). Double-stranded RNA-dependent RNase activity associated with human immunodeficiency virus type 1 reverse transcriptase.. Proceedings of the National Academy of Sciences of the United States of America, 89(3), 927-931.
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2

Multiparameter Flow Cytometry Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained in the presence of 1:500 Fc block (CD16/32, Tonbo) and antibodies diluted 1:200 in FACs buffer (PBS+2.5% FBS) for 45 minutes in the dark at 4°C. Ghost viability dye BV540 (Tonbo) was used to exclude dead cells at 1:500. Cells were fixed in 2% PFA or fixation buffer (Tonbo) for 10–15 at room temperature in the dark prior to data acquisition. Cells were acquired within 24 h using a Cytek Aurora.
Patterson M.T., Burrack A.L., Xu Y., Hickok G.H., Schmiechen Z.C., Becker S., Cruz-Hinojoza E., Schrank P.R., Kennedy A.E., Firulyova M.M., Miller E.A., Zaitsev K., Williams J.W, & Stromnes I.M. (2023). Tumor-specific CD4 T cells instruct monocyte fate in pancreatic ductal adenocarcinoma. Cell reports, 42(7), 112732.
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