Evos m7000 images system

Manufactured by Thermo Fisher Scientific

The EVOS M7000 Imaging System is a high-performance inverted microscope designed for live-cell imaging and time-lapse experiments. It features LED illumination, advanced optics, and a user-friendly software interface for capturing and analyzing images and videos of cells and other microscopic samples.

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Microtome, by Leica (1 mentions)

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EVOS M7000 (107 citations)

3 protocols using evos m7000 images system

Pathological Scoring of Hamster Lung Tissues

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The lung tissues from challenged hamsters were fixed with 10% formalin for 48 h, embedded in paraffin and sectioned. Next, the fixed lung sections were subjected to hematoxylin and eosin (H&E) staining. Whole-slide images of the lung sections were captured by an EVOS M7000 Images System (Thermo Fisher). The standards for the pathological score of lung tissues in this study are adapted and optimized from a recent study of SARS-CoV-2 infection in the hamster model [28] (link). In brief, the H&E staining result of the whole lung tissue was analyzed for its severity of pathological change. The pathological score includes: (a) alveolar septum thickening and consolidation; (b) hemorrhage, exudation, pulmonary edema, and mucous; (c) recruitment and infiltration of inflammatory immune cells. For each issue, score related to the severity (0, no pathological change was observed; 1, moderate pathological change; 2, mild pathological change; 3, indicates severe pathological change; and 4, very severe pathological change). In conclusion, scores of such three issues were added as the comprehensive lung pathological score of lung tissue.

Chen J., Wang P., Yuan L., Zhang L., Zhang L., Zhao H., Chen C., Wang X., Han J., Chen Y., Jia J., Lu Z., Hong J., Lu Z., Wang Q., Chen R., Qi R., Ma J., Zhou M., Yu H., Zhuang C., Liu X., Han Q., Wang G., Su Y., Yuan Q., Cheng T., Wu T., Ye X., Zhang T., Li C., Zhang J., Zhu H., Chen Y., Chen H, & Xia N. (2022). A live attenuated virus-based intranasal COVID-19 vaccine provides rapid, prolonged, and broad protection against SARS-CoV-2. Science Bulletin, 67(13), 1372-1387.

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Histopathological Evaluation of SARS-CoV-2 Infection in Hamster Lungs

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The lung tissues from challenged hamsters were fixed with neutral buffered formalin for 48 hours and processed routinely into paraffin blocks. Then tissues were sectioned to 3 μm by microtome (Leica). Next, the fixed lung sections were stained with hematoxylin and eosin to analyze lung histopathology or were stained with a mouse anti–SARS-CoV-2 NP-specific antibody to evaluate the expression and distribution of viral protein in lung tissues. Whole-slide images of the lung sections were captured with the EVOS M7000 Images System (Thermo Fisher Scientific). Microscopic evaluation of pathological lung lesions was performed blindly by pathologists following a semiquantitative scoring system with the inclusion of three indicators (Yuan et al., 2021 (link)): (i) alveolar septum thickening and consolidation; (ii) hemorrhage, exudation, pulmonary edema, and mucous; and (iii) recruitment and infiltration of inflammatory immune cells. For each hamster, three or four lobes of the lung were assessed independently, and the average score was calculated to indicate the overall pathological severity.

Wu Y., Wang S., Zhang Y., Yuan L., Zheng Q., Wei M., Shi Y., Wang Z., Ma J., Wang K., Nie M., Xiao J., Huang Z., Chen P., Guo H., Lan M., Xu J., Hou W., Hong Y., Chen D., Sun H., Xiong H., Zhou M., Liu C., Guo W., Guo H., Gao J., Gan C., Li Z., Zhang H., Wang X., Li S., Cheng T., Zhao Q., Chen Y., Wu T., Zhang T., Zhang J., Cao H., Zhu H., Yuan Q., Guan Y, & Xia N. (2022). Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine. Cell Host & Microbe, 30(12), 1732-1744.e7.

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Histopathological Lung Tissue Analysis

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The lung tissues from challenged hamsters were fixed with 10% formalin for 48 h, embedded in paraffin and sectioned. Next, the fixed lung sections were subjected to hematoxylin and eosin (H&E) staining. Whole-slide images of the lung sections were captured by an EVOS M7000 Images System (Thermo Fisher). The standards for pathological score of lung tissues in this study are adapted and optimized from a recent study of SARS-CoV-2 infection in hamster model (25 (link)). In brief, H&E staining result of the whole lung tissue was analyzed for its severity of pathological change. The pathological score includes: (i) alveolar septum thickening and consolidation; (ii) hemorrhage, exudation, pulmonary edema, and mucous; (iii) recruitment and infiltration of inflammatory immune cells. For each issue, score related to the severity: 0 indicates no pathological change was observed, 1 indicates moderate pathological change, 2 indicates mild pathological change, 3 indicates severe pathological change, and 4 indicates very severe pathological change. In conclusion, scores of such three issues were added as the comprehensive lung pathological score of a lung tissue.

Chen J., Chen C., Yuan L., Chen Y., Wang X., Tang N., Wei D., Ye X., Xia N, & Chen Y. (2024). Intranasal influenza-vectored COVID-19 vaccines confer broad protection against SARS-CoV-2 XBB variants in hamsters. PNAS Nexus, 3(5), pgae183.

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