Accurel MP1004, a porous polypropylene homopolymer powder with a particle size lesser than 0.4 mm, was purchased from Membrana GmbH (Obenburg, Germany). We purchased p-nitrophenyl palmitate (pNPP) and oleic acid (95%) from Sigma Aldrich (St Louis, Mo, USA). Tripalmitin was acquired from Spectrum Chemicals Mfg. Corp. (Gardena, CA, USA), and the coconut and Spanish extra virgin olive oils were bought from a local market. The reagents Bradford dye, acrylamide, and bisacrylamide were purchased from Biorad (Richmond, CA, USA).
We used the autoinducing medium for growth and expression of the recombinant lipase LipA PSA01: tryptone, yeast extract, lactose, glucose, glycerol, lactose, potassium dihydrogen phosphate, disodium hydrogen phosphate, ammonium chloride, and magnesium sulfate were purchased from PanReac AppliChem (Barcelona, Spain). All solvents (hexane, ethanol, acetone, 2-propanol, ethyl acetate, and methanol) were acquired from JT Baker (Phillipsburg, NJ, USA). Gas chromatography standards and other chemicals used in this study (sodium, sodium hydroxide, TRIS, acetic acid, HEPES) were analytical reagents obtained from Sigma Aldrich (St Louis, Mo, USA).
We used the autoinducing medium for growth and expression of the recombinant lipase LipA PSA01: tryptone, yeast extract, lactose, glucose, glycerol, lactose, potassium dihydrogen phosphate, disodium hydrogen phosphate, ammonium chloride, and magnesium sulfate were purchased from PanReac AppliChem (Barcelona, Spain). All solvents (hexane, ethanol, acetone, 2-propanol, ethyl acetate, and methanol) were acquired from JT Baker (Phillipsburg, NJ, USA). Gas chromatography standards and other chemicals used in this study (sodium, sodium hydroxide, TRIS, acetic acid, HEPES) were analytical reagents obtained from Sigma Aldrich (St Louis, Mo, USA).