Oasis hlb prime cartridges

The methods used for the determination of 46 CECs, including 12 BPs were adapted from Kovačič et al. [12] (link) and Česen et al. [1, (link)13] (link), respectively. All glassware was cleaned and pyrolysed at 400 °C for 4 h. Samples of artificial WW were prepared, as shown in Table SI-2. Briefly, extraction was performed using Oasis HLB Prime cartridges (3 cc, 60 mg, Waters, Massachusetts, USA). After loading and washing the sorbents were dried under vacuum (-1.33 kPa) for 45 min and eluted with the optimal solvent, dried (N2), and derivatised (Table SI-2). The samples were analysed using gas chromatography with mass selective detection (GC-MS, Agilent 7890B/5977A, United States). Separation was achieved using an Agilent Technologies DB-5 MS capillary column (30 m × 0.25 mm x 0.25 µm) with helium (Purity 6.0) as the carrier gas at a flow rate of 1 mL min -1 . Compounds were identified and quantified using selected ion monitoring (SIM) (Table SI-3 and SI-4). Data acquisition and processing were performed using MassHunter software (Agilent Technologies).