AZD6610, AZ12756783, AZ10277797, and AZ13533012 (Barlind et al., 2013) , were supplied by Compound Management, AstraZeneca R&D. Felodipine, dextromethorphan, celecoxib, clozapine and metoprolol were purchased from Sigma-Aldrich (Saint Louis, Missouri, USA) along with NADP + , glucose-6-phosphate and glucose-6-phosphate dehydrogenase.
Methanol (MeOH), acetonitrile optima (ACN), LC/MS grade and Pierce™ formic acid, were obtained from ThermoFisher Scientific (Loughborough, UK). Dimethylsulfoxide (DMSO) was obtained from Sigma-Aldrich (Saint Louis, Missouri, USA). Ultra-pure water was prepared by an in-house water purification system (Milli-Q, Elix® technology Inside, Merck, Darmstadt, Germany). All other chemicals and solvents were of the highest quality commercially available.
Plates used in the incubations were Greiner 96-well microplates, Greiner 384-well microplates from Sigma Aldrich (Saint Louis, Missouri, USA) and Nunc 96-well microplates from ThermoFisher Scientific (Loughborough, UK).
Methanol (MeOH), acetonitrile optima (ACN), LC/MS grade and Pierce™ formic acid, were obtained from ThermoFisher Scientific (Loughborough, UK). Dimethylsulfoxide (DMSO) was obtained from Sigma-Aldrich (Saint Louis, Missouri, USA). Ultra-pure water was prepared by an in-house water purification system (Milli-Q, Elix® technology Inside, Merck, Darmstadt, Germany). All other chemicals and solvents were of the highest quality commercially available.
Plates used in the incubations were Greiner 96-well microplates, Greiner 384-well microplates from Sigma Aldrich (Saint Louis, Missouri, USA) and Nunc 96-well microplates from ThermoFisher Scientific (Loughborough, UK).