Thylakoid membranes were prepared according to Järvi et al., 2011, from Arabidopsis leaves either adapted to LL (125 μmol photons m -2 s -1 ) or HL (1150 μmol photons m -2 s -1 ). 1 μM of chlorophyll from thylakoids was suspended in the fluorescence buffer (60% glycerol, 300 mM sucrose, 5 mM MgCl2, 20 mM HEPES pH 7.8) and measured in 1 cm polymethyl methacrylate cuvettes in a Opistat liquid nitrogen cooled bath cryostat (Oxford Instruments). Fluorescence emission measurements were performed as previously described using a FluoroLog FL3-22 spectrofluorimeter (Jobin Yvon) 32 .
Fluorolog fl3 22 spectrofluorimeter
Manufactured by Horiba
Sourced in France
The FluoroLog FL3-22 spectrofluorimeter is a laboratory instrument designed for fluorescence measurement and analysis. It is capable of exciting a sample with a monochromatic light source and detecting the resulting fluorescent emission.
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Lab products found in correlation
2 protocols using fluorolog fl3 22 spectrofluorimeter
1
Thylakoid Membrane Isolation and Fluorescence Characterization
2
Luminescence Spectroscopy Characterization
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All of the luminescence measurements were carried out at room temperature. We used a Fluorolog FL3-22 spectrofluorimeter (Horiba Jobin Yvon, Longjumeau, France) with double-grating excitation and emission monochromators for luminescence measurements over 400 to 700 nm wavelength range with a 0.1 nm step. PL spectra deconvolutions were carried out with OriginPro 8 SR4 (OriginLab Corp., Northampton, MA, USA) software using the Fit Multiple Peak procedure. The luminescence decay kinetics were studied by the excitation of a pulsed diode laser (λ = 377 nm, Δτ = 1.5 ns) and a Xenon 450W Ushio UXL-450S/O lamp (355 nm). Processing of the luminescence decay curves was carried out using the Fit Exponential procedure of an OriginPro 8 SR4 software. All of the decay curves were described by two exponentials (criterion Adj. R-Square > 0.998). The final data were averaged over 5 measurements.
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