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Phgdh activity assay kit

Manufactured by PromoCell
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The PHGDH Activity Assay Kit is a laboratory tool designed to measure the activity of the enzyme phosphoglycerate dehydrogenase (PHGDH) in biological samples. The kit provides the necessary reagents and protocols to quantify PHGDH enzyme activity using a colorimetric detection method.

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2 protocols using phgdh activity assay kit

1

Metabolic Effects of NCT-503 in Cells

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Cells were plated at 3 × 106 cells per dish in 10 cm dishes and treated with either 10 μM NCT-inactive or 10 μM NCT-inactive with 15 μM NCT-503 for 48 h. PHGDH activity was assessed using cell lysates in the PHGDH Activity Assay Kit (Cat # PK-CA577-K569, PromoCell, Heidelberg, Germany) per manufacturer’s protocol. Extracellular lactate levels were assessed using extracellular medium in the PicoProbe Lactate Fluorometric Assay Kit (Cat # K638, Biovision, Milpitas, CA) per manufacturer’s protocol. Total and serine-derived αKG levels were assessed using cell lysates in the Alpha Ketoglutarate (alpha KG) Assay Kit (Cat # ab83431, Abcam, Cambridge, United Kingdom) per manufacturer’s protocol.
Rathore R., Caldwell K.E., Schutt C., Brashears C.B., Prudner B.C., Ehrhardt W.R., Leung C.H., Lin H., Daw N.C., Beird H.C., Giles A., Wang W.L., Lazar A.J., Chrisinger J.S., Livingston J.A, & Van Tine B.A. (2021). Metabolic compensation activates pro-survival mTORC1 signaling upon 3-phosphoglycerate dehydrogenase inhibition in osteosarcoma. Cell reports, 34(4), 108678.
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2

Metabolic Effects of NCT-503 in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated at 3 × 106 cells per dish in 10 cm dishes and treated with either 10 μM NCT-inactive or 10 μM NCT-inactive with 15 μM NCT-503 for 48 h. PHGDH activity was assessed using cell lysates in the PHGDH Activity Assay Kit (Cat # PK-CA577-K569, PromoCell, Heidelberg, Germany) per manufacturer’s protocol. Extracellular lactate levels were assessed using extracellular medium in the PicoProbe Lactate Fluorometric Assay Kit (Cat # K638, Biovision, Milpitas, CA) per manufacturer’s protocol. Total and serine-derived αKG levels were assessed using cell lysates in the Alpha Ketoglutarate (alpha KG) Assay Kit (Cat # ab83431, Abcam, Cambridge, United Kingdom) per manufacturer’s protocol.
Rathore R., Caldwell K.E., Schutt C., Brashears C.B., Prudner B.C., Ehrhardt W.R., Leung C.H., Lin H., Daw N.C., Beird H.C., Giles A., Wang W.L., Lazar A.J., Chrisinger J.S., Livingston J.A, & Van Tine B.A. (2021). Metabolic compensation activates pro-survival mTORC1 signaling upon 3-phosphoglycerate dehydrogenase inhibition in osteosarcoma. Cell reports, 34(4), 108678.
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