All fluorescence spectra were recorded using a Jasco FP-750 spectrofluorimeter (Kyoto, Japan) equipped with a 150 W xenon lamp using a 1 cm quartz cell. The excitation and emission monochromator bandwidths were 5 nm. All measurements were performed at 25±0.1°C that was controlled using a peltier thermostated cell holder (Jasco, Japan). The pH of solutions was measured with Metrohm 654 pH meter (Herisau, Switzerland).
Peltier thermostated cell holder
Manufactured by Jasco
Sourced in Japan, United States
The Peltier thermostated cell holder is a laboratory equipment used to precisely control the temperature of samples or cells during various scientific experiments and analyses. It utilizes the Peltier effect to heat or cool the sample holder, allowing for accurate temperature regulation.
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Lab products found in correlation
2 protocols using peltier thermostated cell holder
1
Fluorescence Spectra Measurement Protocol
2
CD Spectroscopy of Purified Protein
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CD spectra were recorded using a Jasco
J-1500 spectropolarimeter equipped with a Peltier thermostated cell
holder (Jasco, Easton, MD, United States). Purified ΔN190TTF1
protein was diluted to 15 ng/μL in buffer A (25 mM Tris-Cl at
pH 7.5 and 100 mM KCl). Data collection and analysis were carried
in triplicate as described by Colarusso et al.19 (link) Secondary structure evaluation was performed using the
Jasco20 (link) and Origin software (version 2022,
Origin Lab Corp., Northampton, MA, United States),21 (link) following the standard procedure.22 (link)
J-1500 spectropolarimeter equipped with a Peltier thermostated cell
holder (Jasco, Easton, MD, United States). Purified ΔN190TTF1
protein was diluted to 15 ng/μL in buffer A (25 mM Tris-Cl at
pH 7.5 and 100 mM KCl). Data collection and analysis were carried
in triplicate as described by Colarusso et al.19 (link) Secondary structure evaluation was performed using the
Jasco20 (link) and Origin software (version 2022,
Origin Lab Corp., Northampton, MA, United States),21 (link) following the standard procedure.22 (link)
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