Animals were deeply anesthetized with an i.p. injection of ketamine-xylazine cocktail (16 mg/kg and 80 mg/kg respectively) and perfused intracardially with ice-cold PBS and then with ice-cold 4% paraformaldehyde in PBS. Spinal cords were removed and placed in 4% paraformaldehyde for 2 more hours. Then the tissues were buffered first, in 15% sucrose overnight and then in 30% sucrose solution for another 24 h at 4 °C. Finally, the tissues were embedded in mounting medium Cryoplast (Biopack, Buenos Aires, Argentina) and stored at − 80 °C until sectioning. 20 μm-thick serial sections of lumbar spinal cord were obtained using a cryostat (CM1510 S model, Leica Microsystems, Wetzlar, Germany), attached to aminopropyltriethoxysilane (APES, Sigma #A3648) treated-glass slides, and stored at − 80 °C until use.
Cm1510 s model
Manufactured by Leica
Sourced in Germany
The Leica CM1510 S is a cryostat designed for sectioning frozen tissue samples. It features a motorized specimen head and a high-performance cooling system to maintain the required low temperature for optimal sample preservation during the sectioning process.
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Lab products found in correlation
2 protocols using cm1510 s model
1
Spinal Cord Tissue Preparation for Histology
2
Spinal Cord Tissue Preparation and Cryosectioning
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Animals were deeply anesthetized with an i.p. injection of ketamine-xylazine cocktail (16 mg/kg and 80 mg/kg respectively) and perfused intracardially with ice-cold PBS and then with ice-cold 4% paraformaldehyde in PBS. Spinal cords were removed and placed in 4% paraformaldehyde for 2 more hours. Then the tissues were buffered first, in 15% sucrose overnight and then in 30% sucrose solution for another 24 h at 4°C. Finally, the tissues were embedded in mounting medium Cryoplast (Biopack, Buenos Aires, Argentina) and stored at -80°C until sectioning. 20 μm-thick serial sections of lumbar spinal cord were obtained using a cryostat (CM1510 S model, Leica Microsystems, Wetzlar, Germany), attached to aminopropyltriethoxysilane (APES, Sigma #A3648) treated-glass slides, and stored at -80°C until use.
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