Ta topo cloning kits

Manufactured by Thermo Fisher Scientific

The TA TOPO cloning kits are a set of molecular biology tools designed for the rapid and efficient insertion of PCR amplified DNA fragments into plasmid vectors. The kits provide a simple, rapid, and highly efficient method for the direct cloning of Taq polymerase-amplified PCR products.

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Lab products found in correlation

Taqgold, by Thermo Fisher Scientific (2 mentions) Dna walking speedup kit, by Seegene (1 mentions)

2 protocols using ta topo cloning kits

Genome Walking Amplification and Cloning

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We used Seegene’s DNA walking SpeedUp kit (K1052; Seegene, Rockville, MD) to amplify additional regions of Lei. PCR amplification was performed following Seegene kit protocol using kit primers and gene-specific primers designed for this study. Genome walking PCR products were cloned using TA TOPO cloning kits (45-0641; Invitrogen) and screened using the polymerase TaqGold (Applied Biosystems, CA).

Gao F., Roy S.W, & Katz L.A. (2015). Analyses of Alternatively Processed Genes in Ciliates Provide Insights into the Origins of Scrambled Genomes and May Provide a Mechanism for Speciation. mBio, 6(1), e01998-14.

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Micronuclear Sequence Amplification

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Micronuclear sequences of PKc for USA strain were amplified using Seegene’s DNA Walking SpeedUp™ kit (K1052; Seegene, Rockville, MD). PCR amplification was performed following Seegene kit protocol using kit primers and gene-specific primers designed for this study (Figure S2, Table S1). Genome walking PCR products were cloned using TA TOPO cloning kits (Invitrogen 45–0641), and screened using the polymerase TaqGold (Applied Biosystems, CA).

Gao F., Song W, & Katz L.A. (2014). Genome structure drives patterns of gene family evolution in ciliates, a case study using Chilodonella uncinata (Protista, Ciliophora, Phyllopharyngea). Evolution; international journal of organic evolution, 68(8), 2287-2295.

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