Images were acquired on a spinning disc confocal microscope (Nikon Ti-Eclipse inverted microscope with a Yokogawa CSU-X spinning disk unit and an Andor iXon EM-CCD camera) equipped with a 40 × 0.95 NA air and a 100 × 1.49 NA oil immersion objective. The microscope was controlled using µManager. For TIRF imaging, images were acquired on the same microscope with a motorized TIRF arm using a Hamamatsu Flash 4.0 camera and the 100 × 1.49 NA oil immersion objective.
Csu x spinning disk unit
Manufactured by Oxford Instruments
The CSU-X spinning disk unit is a core piece of lab equipment designed for high-speed confocal microscopy. It utilizes a spinning disk to enable rapid image acquisition, allowing for real-time visualization of dynamic biological processes. The CSU-X provides a compact and efficient solution for researchers in fields requiring advanced imaging capabilities.
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2 protocols using csu x spinning disk unit
1
Spinning Disc Confocal Microscopy Protocol
2
Spinning Disc Confocal Microscopy Imaging
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Images were acquired on a spinning disc confocal microscope (Nikon Ti-Eclipse inverted microscope with a Yokogawa CSU-X spinning disk unit and an Andor iXon EM-CCD camera) equipped with a 40 × 0.95 NA air and a 100 × 1.49 NA oil immersion objective. The microscope was controlled using µManager. For TIRF imaging, images were acquired on the same microscope with a motorized TIRF arm using a Hamamatsu Flash 4.0 camera and the 100x 1.49 NA oil immersion objective.
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