SDS-PAGE and NativePage gels were stained with Brilliant Blue G-250 or R-250 (CBB; Bio-Rad), respectively. Alternatively, proteins were transferred to nitrocellulose (SDS-PAGE) or PVDF (BN-PAGE) membranes. Proteins were visualized using standard immunodetection techniques. Primary antibodies used in this study were anti-GroEL2 (CS44; Colorado State University), anti-PE_PGRS antibody (7C4.1F7) (77 (link)), anti-EsxA (Hyb76-8) (78 (link)), polyclonal anti-FtsH (79 (link)), polyclonal anti-PPE68 (9 (link)), polyclonal anti-EspE (51 (link), 52 (link)), and polyclonal anti-Strep (Novusbio).
Brilliant blue g 250
Manufactured by Bio-Rad
Sourced in United States
Brilliant Blue G-250 is a protein-binding dye used in various biochemical applications. It is a dark blue crystalline powder that is soluble in water and forms a blue-colored solution. The dye binds to proteins and is commonly used in protein assays, such as the Bradford assay, for the quantitative determination of protein concentrations.
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Lab products found in correlation
Sodium pyrophosphate, by Merck Group (1 mentions)
Sodium chloride (nacl), by Duksan Pure Chemicals (1 mentions)
Sodium deoxycholate, by Merck Group (1 mentions)
Mes hydrate, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Duksan Pure Chemicals (1 mentions)
Magnesium chloride, by Merck Group (1 mentions)
Hplc grade water, by Duksan Pure Chemicals (1 mentions)
Hplc grade methanol, by Duksan Pure Chemicals (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Sodium bisulfite, by Merck Group (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Protein assay kit, by Bio-Rad (1 mentions)
Acetic acid, by Tedia (1 mentions)
Hydrochloric acid (hcl), by Duksan Pure Chemicals (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
2 4 6 trinitrobenzenesulfonic acid, by Merck Group (1 mentions)
Bis acrylamide, by Bio-Rad (1 mentions)
Tricine, by Bio-Rad (1 mentions)
3 protocols using brilliant blue g 250
1
Antibody-Based Protein Detection Techniques
2
Analytical Reagents for Protein Analysis
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HPLC-grade acetonitrile (ACN), HPLC-grade water, HPLC-grade methanol (MeOH), hydrochloric acid (HCl), and sodium chloride (NaCl) were obtained from DUKSAN (Kyungkido, Korea). Acetic acid was purchased from TEDIA (Fairfield, OH, USA). Brilliant Blue G-250 and a Bio-Rad protein assay kit were purchased from Bio-Rad (Hercules, CA, USA), and Complete Protease inhibitor cocktail was purchased from Roche (Mannheim, Germany). Other reagents, including 2-mercaptoethanol (β-ME), ammonium bicarbonate (ABC), ammonium persulfate (APS), EDTA, iodoacetamide (IAA), magnesium chloride, MES hydrate, potassium chloride, sodium bisulfite, sodium deoxycholate (SDC), sodium pyrophosphate, and trifluoroAcetic acid (TFA) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Antioxidant Capacity Determination Protocol
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Bovine-serum albumin, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), casein, 2,4,6-trinitrobenzenesulfonic acid (TNBS), L- cysteine, potassium persulfate, tris(hydroxymethyl)aminomethane (Tris), 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH), and 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox) were purchased from Sigma Chemical Company (St. Louis, MO, USA); Coomasie Brilliant Blue G-250, acrylamide, bis-acrylamide, low-range molecular-weight standards (phosphorylase B, serum albumin, ovalbumin, carbonic anhydrase, trypsin inhibitor, and lysozyme: 97.4, 66.2, 45.0, 31.0, 21.5, and 14.4 kDa, respectively) , and tricine from Bio-Rad Hercules, CA, USA); trifluoroacetic acid (TFA) from J.T. Baker (Philipsburg, NJ, USA); L-leucine and trichloroacetic acid from Carlo Erba Reagenti (Rodano, MI, Italy); and Sephadex G-25 fine from GE Healthcare Life Sciences (Uppsala, Sweden). All other chemicals were obtained from local commercial sources and were of the highest purity available.
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