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2 protocols using pluronic f68

1

Encapsulation of 6-Mercaptopurine in PLGA Nanoparticles

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In this study, 6-mercaptopurine (assay purity 98.0%) was purchased from Toronto Research Chemicals Inc (Toronto, Canada). PLGA with a 50/50 for lactic/glycolic ratio (Resomer® RG 503H, acid terminated, molecular weight (Mw) 24,000–38,000; Resomer® RG 502H, acid terminated, Mw 7000–17,000; Resomer® RG 502, ester terminated, Mw 7000–17,000), Polyvinyl alcohol (PVA, 87%–89% hydrolyzed, Mw 31,000–50,000), dichloromethane (DCM), ethyl acetate (EA) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich Chemical Co., Ltd. Pluronic F68 was purchased from Yuanye Bio-Technology Co., Ltd. Ammonia water (NH3.H2O) was obtained from Macklin Co., Ltd (Shanghai, China). Phosphate buffered saline (PBS) and penicillin-streptomycin were obtained from Solarbio Technology Co., Ltd. In addition, cell culture media RPMI1640 and trypsin were bought from Macgene (Beijing, China). Fetal bovine serum (FBS) was supplied by Gibco Invitrogen Co. (Carlsbad, USA). The Cell Counting Kit-8 (CCK8) was purchased from Dojindo (Shanghai, China). The Annexin V-FITC/PI Apoptosis Detection Kit was bought from YEASEN (Shanghai, China). All other reagents were of analytical grade.
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2

Nanomaterial-based Cisplatin Delivery

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Tannic acid (TA), Pluronic® F68, cisplatin (CDDP) and Rhodamine B (RB) were purchased from Yuanye biotechnology company (Shanghai, China). Silver nitrate was obtained from aladdin reagent Co., Ltd. (Shanghai, China). Sodium Dodecyl Sulfonate (SDS) was bought from Sigma-Aldrich (USA). Phosphorylated H2AX (γH2AX) antibody was supplied by Cell Signaling Technology company. NOX4, and NAPDH antibody were purchased from Proteintech company. H2O2 fluorescent probe BES H2O2-Ac was got from Wako (Cat. #021-17801 (1 mg), Japan). MQAE probe and Fe2+ probe (Ferro Orange) were bought from Beyotime company (Shanghai, China) and Dong Ren company (Shanghai, China), respectively.
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