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Anti human cd52

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Anti-human CD52 is a monoclonal antibody that recognizes the CD52 antigen expressed on the surface of human lymphocytes, monocytes, and other hematopoietic cells. It can be used for the identification and enumeration of CD52-positive cells in flow cytometry applications.

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Lab products found in correlation

Facsaria 3, by BD (2 mentions) Rhil 2, by Thermo Fisher Scientific (2 mentions) Mem alpha, by Sartorius (2 mentions) Anti human cd70, by BioLegend (2 mentions) Lymphocyte separation medium, by MP Biomedicals (2 mentions) Heat inactivated fcs, by Merck Group (2 mentions)

2 protocols using anti human cd52

1

Fluorescence-Activated Cell Sorting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell sorting was performed 3 days following electroporation. Cells were harvested, washed and resuspended in PBS supplemented with 5% BSA containing 1/100 diluted anti-human CD70 and anti-human CD52 (Biolegend). Staining was performed for 15 mins at room temperature in the dark. Subsequently, cells were washed and resuspended in PBS supplemented with 0.5% BSA. During Fluorescence Activated Cell Sorting, cells were collected in MEM-Alpha (Biological Industries) supplemented with 0.5% Heat Inactivated FCS (Sigma) and P/S. Acquisition was performed on a BD FACSAria III (BD Biosciences). After collection, live cells were purified using Lymphocyte Separation Medium (mpbio), washed and cultured in MEM-Alpha (Biological Industries) supplemented with 10%Heat Inactivated FCS (Sigma), 50IU rhIL-2 (Peprotech) and P/S. Purity of the sorted cells can be found in Extended Data Fig. 10.
Nahmad A.D., Reuveni E., Goldschmidt E., Tenne T., Liberman M., Horovitz-Fried M., Khosravi R., Kobo H., Reinstein E., Madi A., Ben-David U, & Barzel A. (2022). Frequent Aneuploidy in Primary Human T Cells after CRISPR-Cas9 cleavage. Nature biotechnology, 40(12), 1807-1813.
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2

Fluorescence-Activated Cell Sorting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell sorting was performed 3 days following electroporation. Cells were harvested, washed and resuspended in PBS supplemented with 5% BSA containing 1/100 diluted anti-human CD70 and anti-human CD52 (Biolegend). Staining was performed for 15 mins at room temperature in the dark. Subsequently, cells were washed and resuspended in PBS supplemented with 0.5% BSA. During Fluorescence Activated Cell Sorting, cells were collected in MEM-Alpha (Biological Industries) supplemented with 0.5% Heat Inactivated FCS (Sigma) and P/S. Acquisition was performed on a BD FACSAria III (BD Biosciences). After collection, live cells were purified using Lymphocyte Separation Medium (mpbio), washed and cultured in MEM-Alpha (Biological Industries) supplemented with 10%Heat Inactivated FCS (Sigma), 50IU rhIL-2 (Peprotech) and P/S. Purity of the sorted cells can be found in Extended Data Fig. 10.
Nahmad A.D., Reuveni E., Goldschmidt E., Tenne T., Liberman M., Horovitz-Fried M., Khosravi R., Kobo H., Reinstein E., Madi A., Ben-David U, & Barzel A. (2022). Frequent Aneuploidy in Primary Human T Cells after CRISPR-Cas9 cleavage. Nature biotechnology, 40(12), 1807-1813.
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