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2 protocols using benzophenone

1

Fabrication and Characterization of Functional Hydrogels

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Hydroxyethyl methacrylate (HEMA), ethylene glycol dimethacrylate (EGDMA), lysozyme (chicken egg lysozyme), and BSA were obtained from Sigma Aldrich (St Louis, MO, USA). 2-Methacryloyloxyethyl phosphorylcholine (MPC) was obtained from KCI Limited (Seoul, Korea). DifcoTM Mueller-Hinton broth medium was purchased from Thermo Fisher Scientific (Leicestershire, UK), while azobisisobutyronitrile (AIBN) and benzophenone were procured from Junsei (Tokyo, Japan) and Daejung Chemical & Metal Co. (Shiheung, Korea), respectively. Acetone, trifluoroacetic acid, and other solvents were of analytical grade and used without further purification. The transmittance spectra of the hydrogels were measured at 300–700 nm, in triplicate, using a Shimadzu ultraviolet–visible spectrophotometer 1601 PC (UV-1650PC) (Tokyo, Japan).
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2

Compound Screening for Biological Activity

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Dimethyl sulfoxide (DMSO, 49), p-nitrosodimethylaniline, imidazole, nitroblue tetrazolium (NBT), quinine HCl (1), 4-methyl-7-ethoxycoumarin (3), 7-methoxycoumarin (5), 8-methoxypsoralen (6), acridine (7), diclofenac Na (11), hexachlorophene (16), indomethacin (19), ketoprofen ( 21), methyl β-naphthyl ketone (22), piroxicam (29), promethazine HCl (31), sulfanilamide (34), tetracycline HCl (35), 1,3-butylene glycol (47), 2-propanol (48), ethanol (50), and glycerin (51) were obtained from Fujifilm-Wako Pure Chemical Industries. Sulisobenzone (2), bithionol (9), doxycycline HCl (12), enoxacin (13), furosemide ( 14), glibenclamide (15), hydrochlorothiazide (16), ibuprofen (18), isoniazid (20), mequitazine (24), nalidixic acid (25), octyl dimethyl PABA (26), ofloxacin ( 27), omadine Na (28), prochlorperazine maleate (30), pyridoxine HCl (32), sparfloxacin (33), lactic acid (52), and penicillin G (54) were purchased from Sigma-Aldrich Japan. Benzophenone (8), lauric acid (53), and propylene glycol (55) were obtained from Junsei Chemical Co. (Tokyo, Japan), and 6-methylcoumarin (4) was purchased from Nacalai Tesque. Chlorpromazine HCl (10) and methyl-N-methylanthranilate (23) were purchased from Tokyo Chemical Industry (Tokyo, Japan).
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