T buthylmethylether

Docetaxel (USP 30, grade 99.0%) and paclitaxel (USP 28, grade >99.5%) were supplied by 21CECpharm (London, UK). Poly(methyl vinyl ether-co-maleic anhydride) or poly(anhydride) (PMV/MA) [Gantrez® AN 119; MW 200, 000; density: 1.03 g/mL] was purchased from International Specialty Products ISP/Ahsland Inc. (KY, USA). Taxotere® from Sanofi-Aventis was provided by the Pharmacy Service of University Clinic of Navarra (Pamplona, Spain).
Phosphate buffered saline (PBS), pancreatin and glycine were obtained from Sigma Aldrich (MO, USA). Poly(ethylene glycol) with MW of 2000 and 6000 Da (PEG2000 and PEG6000, respectively) and disodium edetate (EDTA) were provided by Fluka (Buchs, Switzerland).
Pepsin, acetone, ethanol, t-buthylmethylether and acetonitrile were obtained from Merck (Darmstadt, Germany). Polysorbate 80 (Tween® 80) was supplied by Panreac (Barcelona, Spain). Deionised reagent water (18.2 MΩ resistivity) was prepared by a water purification system (Wasserlab, Pamplona, Spain). The anaesthetic isoflurane (Isoflo®) was from laboratories Esteve (Barcelona, Spain). All others reagents and chemicals used were of analytical grade.

Poly(methyl vinyl ether-co-maleic anhydride) or poly(anhydride) (PMV/MA) [Gantrez® AN 119; MW 200, 000] was purchased from International Specialty Products ISP/Ahsland Inc (KY, USA). Docetaxel (USP 30, grade 99.0%) and paclitaxel (USP 28, grade >99.5%) were supplied by 21CECpharm (London, UK). Taxotere®, from Sanofi-Aventis, was provided by the Pharmacy Service of University Clinic of Navarra (Pamplona, Spain). Phosphate buffered saline (PBS), pancreatin, methoxy poly(ethylene glycol) 2000 (mPEG2000) and solid iodine (≥99.8%) were obtained from Sigma Aldrich (MO, USA). Poly(ethylene glycol) with MW of 2000 Da (PEG2000) was provided by Fluka (Buchs, Switzerland). Acetone, ethanol, t-buthylmethylether, acetonitrile and tetrahydrofuran (THF) were obtained from Merck (Darmstadt, Germany). Polysorbate 80 (Tween® 80) was supplied by Panreac (Barcelona, Spain). Deionised reagent water (18.2 MΩ resistivity) was prepared by a water purification system (Wasserlab, Pamplona, Spain). The anaesthetic isoflurane (Isoflo®) was from laboratories Esteve (Barcelona, Spain). All other reagents and chemicals used were of analytical grade and supplied from Sigma Aldrich (MO, USA) and Merck (Darmstadt, Germany).