Complete blood count and biochemical values were compared during the pre-treatment phase, as well as between the second and sixth weeks of the treatment period. The evaluated hematological parameters were packed cell volume (PCV), white blood cell count (WBC), neutrophil count, monocyte count, and lymphocyte count, determined using a Sysmex XN-1000TM Hematology Analyzer (Sysmex, Mundelein, IL, USA). Serum biochemistry parameters were blood urea nitrogen (BUN), creatinine (Creat), alanine aminotransferase (ALT), total protein (TP), and albumin (ALB), determined using an IL Lab 650 chemistry system (Diamond Diagnostics, Holliston, MA, USA). Screening for retroviral infection (FeLV antigen and FIV antibody) was performed using a rapid immune migration-based (WITNESS ® FeLV-FIV) point-of-care test kit.
Xn 1000 hematology analyzer
Manufactured by Sysmex
Sourced in Japan, United States, Germany
The XN-1000 is a hematology analyzer designed for clinical laboratory use. It is capable of performing complete blood count (CBC) and differential analysis on blood samples. The XN-1000 uses advanced technology to provide accurate and reliable results for a wide range of hematological parameters.
Automatically generated - may contain errors
Lab products found in correlation
Il lab 650 chemistry system, by Diamond Diagnostics (2 mentions)
Vitros 350 autoanalyzer, by Johnson & Johnson (1 mentions)
Brightspeed 16 ct scanner, by GE Healthcare (1 mentions)
Immunocap, by Thermo Fisher Scientific (1 mentions)
Phadia 250 system, by Thermo Fisher Scientific (1 mentions)
Enzyme linked immunosorbent assay, by R&D Systems (1 mentions)
Xn 9000, by Sysmex (1 mentions)
Chemiluminescent enzyme immunoassay, by Fujirebio (1 mentions)
Au680 chemistry analyzer, by Beckman Coulter (1 mentions)
Cobas 6000 c501, by Roche (1 mentions)
Bio plex 200 system, by Bio-Rad (1 mentions)
Cobas e411, by Roche (1 mentions)
Cobas 8000 c702 analyzer, by Roche (1 mentions)
Acl top 750 cts, by Werfen (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Trucount, by BD (1 mentions)
Nuclisens easyq hiv 1 v2.0 kit, by bioMérieux (1 mentions)
Edta anticoagulant vacutainer tubes, by BD (1 mentions)
Vitros 5600, by Ortho Clinical Diagnostics (1 mentions)
K3 edta tubes, by Sarstedt (1 mentions)
47 protocols using xn 1000 hematology analyzer
1
Hematological and Biochemical Profiles in Feline Patients
2
Mouse Whole Blood Collection and Hematology
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At the designated endpoint, whole blood was collected from anesthetized mice into K3-EDTA BD Microtainer MAP Microtubes (Thermo Fisher Scientific, 22–253–270) by cardiac puncture. Hematology analysis was performed with a Sysmex XN-1000TM hematology Analyzer (Sysmex America Inc.).
3
Comprehensive Blood Biomarker Profiling
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Overnight-fasting blood samples were collected in EDTA tubes and processed immediately for general biochemistry and hematological analysis. These were performed by the analysis unit of Puerta del Mar University Hospital and included fibrinogen (mg/dL) using the ACL-TOP® CTS300 analysis system (Werfen, Spain), C-reactive protein (mg/L), lactate dehydrogenase (IU/mL) using the Alinity™ analysis system (Abbot, Spain) and angiotensin-converting enzyme (IU/L) using the BA-200 analysis system (Biosystem, Spain). Leukocyte populations were analyzed using an automated XN-1000 hematology analyzer (Sysmex, Germany), and the NLR, PLR, LMR SII, SIRI and AISI were calculated.
4
Clopidogrel's Effect on Platelet Levels in Hip Fractures
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The platelet level of patients with hip fracture was measured when the patients were admitted in our hospital and detected by XN‐1000 hematology analyzer (Sysmex Corporation, Kobe, Japan). This study was conducted in the patients taking clopidogrel, and the drug was an anti‐platelet agent. The platelet level was measured as to evaluate the effects of clopidogrel on the bleeding conditions.
5
Hemoglobin Concentration and Parkinsonism
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Serum hemoglobin concentration (Field ID 30020) was measured at the central processing laboratory of the UK Biocenter. A median of one measurement was obtained per subject. Hemoglobin concentration was measured in EDTA-anticoagulated blood using the Sysmex XN-1000 hematology analyzer. The descriptions in this subsection are reproduced from a previous study using the same data [21 (link)].
Associations with other variables such as incident parkinsonism were analyzed in three ways. First, serum hemoglobin concentration was included as a continuous variable. Second, concentrations were stratified into 5 quintiles. Third, we divided concentrations into anemia, normal range, and polycythemia according to criteria of World Health Organization [22 ,23 (link)]: anemia (women: <115 g/L, men: <130 g/L), polycythemia (women: >160 g/L, men: >165 g/L). The latter two methods were used to investigate the nonlinearity of the association between hemoglobin concentration and risk of incident parkinsonism.
Associations with other variables such as incident parkinsonism were analyzed in three ways. First, serum hemoglobin concentration was included as a continuous variable. Second, concentrations were stratified into 5 quintiles. Third, we divided concentrations into anemia, normal range, and polycythemia according to criteria of World Health Organization [22 ,23 (link)]: anemia (women: <115 g/L, men: <130 g/L), polycythemia (women: >160 g/L, men: >165 g/L). The latter two methods were used to investigate the nonlinearity of the association between hemoglobin concentration and risk of incident parkinsonism.
6
Quantifying Blood MMP-8 and MMP-9 Levels
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Blood was drawn in EDTA tube and sent to the hospital central laboratory. Complete blood count was tested (Sysmex XN-1000 hematology analyzer). Neutrophils and lymphocytes proportion and absolute count information was extracted from the laboratory report.
Using separated plasma from patient’s whole blood collected in plain tube, matrix metalloproteinase (MMP) −8 and −9 were examined using ELISA method, following the instruction manual from the company (R&D System® Elisa Kit cat no. DY908 and DY911, respectively). To determine the optimal ELISA result, we conducted several testing in serial plasma concentration using PBS as dilution. MMP-8 were measured at concentration dilution of 8 times, whereas MMP-9 were measured at concentration dilution of 128 times.
Using separated plasma from patient’s whole blood collected in plain tube, matrix metalloproteinase (MMP) −8 and −9 were examined using ELISA method, following the instruction manual from the company (R&D System® Elisa Kit cat no. DY908 and DY911, respectively). To determine the optimal ELISA result, we conducted several testing in serial plasma concentration using PBS as dilution. MMP-8 were measured at concentration dilution of 8 times, whereas MMP-9 were measured at concentration dilution of 128 times.
7
Omicron Variant Hospitalization and Treatment
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COVID-19 patients infected by the omicron variant of SARS-CoV-2 were hospitalized and treated as reported (17 (link)). Blood cell analysis was conducted by an automated XN1000 hematology analyzer (SYSMEX, Japan), and biochemical indicators were analyzed using VITROS 350 autoanalyzer (Johnson &. Johnson, USA). Computed tomography (CT) was performed using BrightSpeed 16 CT Scanner (GE Healthcare, USA). The scanning parameters were set as 120 kVp, 80 mA, 1.5-mm collimation, reconstruction matrix of 512 × 512, slice thickness of 5.0 mm, scan field of view (FOV) of 25 × 25 cm, and high spatial resolution algorithm. For most of admitted COVID-19 patients in TFPHS, two types of inactivated vaccines (Sinovac or Sinopharm) have been administered. Serological tests of patients based on detection of SARS-CoV-2-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) were conducted, using 2019-nCoV Ab test kit (colloidal gold), manufactured by Innovita Biological Technology Co. Ltd., China.
8
Measuring Hemoglobin in UK Biobank Samples
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Among samples collected at recruitment, hemoglobin concentrations (Field ID 30020, mg/dL) were measured in EDTA‐vacutainers using the Sysmex XN‐1000 hematology analyzer at the laboratory of the UK Biocenter (https://biobank.ctsu.ox.ac.uk/crystal/crystal/docs/haematology.pdf ).
35 (link),
36 (link) The primary outcomes included incident cases of CAD, HF, IS, and T2D, which were identified through linkage to Hospital Episodes Statistics and mortality data, with diagnostic criteria defined by the International Classification of Diseases, Ninth Revision (ICD‐9) and Tenth Revision (ICD‐10) codes (TableS2 ). Data from UKB are available to bona fide researchers on application to https://www.ukbiobank.ac.uk . Data from other genetic consortia are also available on application to the relevant consortia.
35 (link),
36 (link) The primary outcomes included incident cases of CAD, HF, IS, and T2D, which were identified through linkage to Hospital Episodes Statistics and mortality data, with diagnostic criteria defined by the International Classification of Diseases, Ninth Revision (ICD‐9) and Tenth Revision (ICD‐10) codes (Table
9
Clostridium difficile Infection Epidemiology
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Clinical data, including sex, age, nasogastric tube use, underlying diseases, and laboratory data, were collected from electronic medical records using a predetermined record form. Medications, including antibiotics, proton pump inhibitors, histamine H2-receptor antagonists, or steroids, prescribed within one month before the onset of CDI or during hospitalization were recorded. The place of CDI onset, such as wards or intensive care units (ICUs), was recorded. Chronic kidney disease (CKD) was defined as an estimated glomerular filtration rate (eGFR) of < 60 mL/min/1.73 m2 for at least three months.15 (link) Leukocyte counts and differential ratios within two days after CDI onset were recorded. Leukocyte counts and differential ratios were analyzed using a Sysmex XN-1000™ Hematology Analyzer (Sysmex@, Canada). Colonization or infection of vancomycin-resistant enterococci (VRE) during hospitalization was recorded based on medical charts.
10
Neonatal Outcome Factors in Preterm Infants
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Birth weight, gestational age, volume of blood transfusion, duration of oxygen supplementation, culture-proven sepsis, intraventricular haemorrhage, enterocolitis, congenital heart disease, pneumonia, gender, ROP stage, requirement for ROP treatment, and weekly mean haemoglobin levels were collected from birth until week six of life. Haemoglobin levels were measured using the Automated Blood Cell Analyzer (Sysmex XN-1000™ Hematology Analyzer), with 500 microLiters of blood per phlebotomy collected in a K2EDTA tube. Culture-proven sepsis was defined as one episode of positive blood culture during the first six weeks of life. ROP was staged based on the revised ‘International Classification of Retinopathy of Prematurity’ [21 (link),22 (link)]. This classifies ROP into 5 stages; Stage 1—a demarcation line, i.e., a line separating the avascular and vascularised retina; Stage 2—a ridge; Stage 3—extraretinal fibrovascular proliferation; Stage 4—partial retinal detachment, extrafoveal (Stage 4a) or foveal (Stage 4b); and Stage 5—total retinal detachment. Treatment was based on recommendations from the ‘Early Treatment For Retinopathy of Prematurity Randomized Trial’ [23 (link)].
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