CRC cell lines HT-29, CT-26, SW480, SW620, HT-29, HCT-116, SW1116 and normal colorectal epithelial cell line NCM460 were purchased from Keygen (Nanjing, China); Oxaliplatin resistant HCT-116-OR cells were purchased from Shanghai Meixuan Biological Technology Co., LTD. Cell lines were passaged in our laboratory. Cell line authentication was assessed using short tandem repeat (STR) DNA profiling method every 6 months. All of these cell lines were cultured in RPMI-1640 medium (Keygen) with 10% Fetal bovine serum (FBS, Biological Industries, Kibbutz Beit Haemek, Israel) at 37°C plus 5% CO2. Recombinant human TGF beta Receptor II (rTGFBR2) protein was purchased from Abcam (Cambridge, MA, USA) and 50 nM was used in this study.
Sw1116
Manufactured by Keygen Biotech
Sourced in China
The SW1116 is a specialized lab equipment designed for cell culture applications. It is a water-jacketed incubator that provides precise control over temperature, humidity, and carbon dioxide levels to support the optimal growth and maintenance of cell lines. The SW1116 is a reliable and versatile tool for researchers and scientists working in the field of cell biology and biotechnology.
Automatically generated - may contain errors
Lab products found in correlation
Sw480, by Keygen Biotech (3 mentions)
Hct116, by Keygen Biotech (2 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Sw620, by Keygen Biotech (1 mentions)
Ncm460, by Keygen Biotech (1 mentions)
Rpmi 1640 medium, by Keygen Biotech (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Hct 8, by Keygen Biotech (1 mentions)
Caco 2, by Keygen Biotech (1 mentions)
Hct15, by Keygen Biotech (1 mentions)
3 protocols using sw1116
1
Colorectal Cancer Cell Line Culturing
2
Colon Cancer Cell Line and Tissue Sample Collection
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Human CRC cell lines SW1116 and SW480 (purchased from Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and HEK-293T cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc.), 100 IU/ml penicillin and 100 mg/ml streptomycin. All cells were incubated at 37°C in an atmosphere of 5% CO2.
Colon cancer tissues and adjacent normal tissues were collected from clinical patients undergoing colon cancer resection. All tissue samples were immediately snap-frozen in liquid nitrogen following surgery. All human CRC samples were divided into Grade I, Grade II and Grade III–IV according to the WHO classification (24 (link)). In total, 50 pairs of CRC tissues and adjacent normal tissues from patients who underwent surgical operations at The Third Affiliated Hospital of Soochow University (Changzhou, China) from August 1, 2013 to July 31, 2014, were obtained for the study. Written informed consent was obtained from all patients. The present study was approved by the review board and ethics committee of The Third Affiliated Hospital of Soochow University.
Colon cancer tissues and adjacent normal tissues were collected from clinical patients undergoing colon cancer resection. All tissue samples were immediately snap-frozen in liquid nitrogen following surgery. All human CRC samples were divided into Grade I, Grade II and Grade III–IV according to the WHO classification (24 (link)). In total, 50 pairs of CRC tissues and adjacent normal tissues from patients who underwent surgical operations at The Third Affiliated Hospital of Soochow University (Changzhou, China) from August 1, 2013 to July 31, 2014, were obtained for the study. Written informed consent was obtained from all patients. The present study was approved by the review board and ethics committee of The Third Affiliated Hospital of Soochow University.
3
Colorectal Adenocarcinoma Cell Lines Profiled
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The following colorectal adenocarcinoma cell lines with STR profiling, were obtained from KeyGEN BioTECH, Jiangsu: HCT8 (male), HCT15 (male), HCT116 (male), LoVo (lymph node metastasis, male), SW480 (male), SW1116 (male), HT29 (female), Caco-2 (male), DLD-1 (male), and T84 (lung metastasis, male). The DiFi cell line was a generous gift of Dr. Li Luo. Cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium supplemented with 10% fetal bovine serum (FBS), penicillin (100 mg/ml) and streptomycin (100 mg/ml), and cells were subcultured after dissociation with 0.25% trypsin-EDTA once they had reached 80% confluence. In general, all cultures were maintained in a 37 °C 5% CO2 incubator, or incubated in a hypoxia chamber at 94% N2, 5% CO2, and 1% O2 for certain experiments.
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