Mcf 7

PC-3, MCF-7, HeLa, U87, HPAEpiC, and A549 cells were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China); PC-3, MCF-7, HeLa, U87, and A549 cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% v/v fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin; HPAEpiC cells were cultured in RPMI 1640 Medium supplemented with 10% v/v fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. All cells were at 37°C in a humidified atmosphere of 5% CO₂.

The human breast cancer cell lines MCF-7, MDA-MB-231, and MCF 10A were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). This study presents data on GPD1 expression and survival rates by distinguishing between patients with Luminal, HER2 positive, and triple-negative breast cancer (TNBC). The MCF-7 and MDA-MB-231 cell lines used were also derived from Luminal and triple-negative patients, respectively. All cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (11965, Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (10100, Gibco, USA), 100 U/ml penicillin G and 100 μg/mL streptomycin at 37 °C in a 5% CO₂ humidified incubator [18 (link)].
The clinical samples of human breast cancer tissues and the corresponding adjacent benign tissues from the same patients were collected from Henan Provincial People's Hospital. The inclusion criterion was that the patient did not receive chemotherapy or radiotherapy before surgery. All patients were diagnosed and graded by pathologists to ensure the accuracy of clinical specimens. This study was approved by the Ethics Committee of Henan Provincial People's Hospital (Approval number: 2021-84). Informed consent was obtained from all subjects involved in the study.

Human cell lines HCT116, 786-O and A549 were purchased from Hunan Fenghui Biotechnology Co., Ltd. (Changsha, China). Cell lines SW480, MCF-7 and Huh7 were purchased from National Collection of Authenticated Cell Cultures (Shanghai, China). THP-1 was purchased from Fuzhou Zolgene Biotechnology Co., Ltd. (Fuzhou, China). HCT116 and 786-O were cultured in RPMI1640, A549 in DMEM/F12, SW480, MCF-7 and Huh7 in DMEM. THP-1 was cultured in RPMI1640 with 0.05 mM β-mercaptoethanol. These media were supplemented with 10% FBS, 1% antibiotics, and cells maintained in a 37 ℃ incubator with 5% CO₂. NSC146109 (HY-108638, MedChemExpress, USA) and Oxaliplatin (OXA) were dissolved in DMSO at a final concentration of 10 mM and stored at − 20 ℃. The stock was diluted to the required concentration with corresponding medium when needed.
Cells were seeded in a 96-well plate with 8000 cells per well. The next day, cells were treated with various concentration of NSC146109 or OXA for 48 h. Before measuring the OD value in 450 nm, 10% CCK8 was added and cells were cultured in a 37 ℃ incubator for 1 h.