Hematoxylin and eosin (H&E)-stained sections of the tumor were blindly reviewed by three experienced pulmonary pathologists. Immunohistochemical (IHC) staining was performed to exclude mixed and inconspicuous ADC components. The lung tissue sections were deparaffinized three times with xylene and dehydrated through a graded series of ethanol. Endogenous peroxidase activity was quenched with 3% H2O2 in water for 10 min. Antigen retrieval was performed by heating the slides in 0.1 M sodium citrate (pH 6.0) for 10 min. The sections were then incubated with primary antibodies for 30 min at room temperature. Sections incubated with antibody diluents were used as negative controls. The sections were developed using the Dako EnVision™ visualization system (Dako Cytomation, CA, USA), and the following antibodies were used for IHC staining: ΔNP63 (p40; Calbiochem, Darmstadt, Germany) and cytokeratin 5/6 (CK5/6; Dako).
Dako envision visualization system
Manufactured by Agilent Technologies
Sourced in United States
The Dako EnVision™ visualization system is a laboratory equipment used for the detection and visualization of specific target molecules in tissue samples. It provides a reliable and efficient method for immunohistochemical and in situ hybridization analyses.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dako envision visualization system
1
Immunohistochemical Analysis of Lung Adenocarcinoma
2
Immunohistochemical Examination of Lung Cancer
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TTF-1 (Clone 8G7G3/1, DakoCytomation, Glostrup, Denmark), CK7 (Clone OV-TL 12/30, DakoCytomation), ΔNP63 (P40, Calbiochem, California, USA) and high molecular cytokeratin (Clone 34βE12, DakoCytomation) were used for examination of the pathological histology that had been identified through H&E staining to exclude mixed and inconspicuous, adenocarcinoma components. Tissue sections were deparaffinized using xylene and dehydrated in alcohol. Antigen-retrieval was performed by heating the slides in 0.1 M sodium citrate (pH 6.0) for 10 min. Tissue slides were incubated in 0.3% H2O2 for 10 min to block endogenous peroxidase activity. Sections were incubated with primary antibody for 30 min at room temperature. Sections incubated with antibody diluents (Immunologic, Duiven, Netherlands) were used as the negative control. Sections were developed using the Dako EnVision™ visualization system (DakoCytomation). Pretreatment conditions and antibody dilutions are available upon request.
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