Rac1 antibody

Manufactured by Santa Cruz Biotechnology

The Rac1 antibody is a research tool used to detect the Rac1 protein, a small GTPase involved in various cellular processes. It can be used in techniques such as Western blotting, immunoprecipitation, and immunocytochemistry to study the expression and localization of Rac1 in biological samples.

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Lab products found in correlation

2 protocols using rac1 antibody

Comprehensive Immunological Assay Protocol

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Rac2 antibody is from Novus Biologicals. Rac1 antibody is obtained from Santa Cruz Biotechnology. Vitronectin and collagen are from Sigma (Sigma-Aldrich, St. Louis, MO), fragments of fibronectin (H296 and CH271) are from R&D systems. Primary or fluorescent antibodies against CD31 (clone MEC13.3), CD11b (clone M1/70) are from BD Biosciences, F4/80 (clone BM8) is from eBiosciences. 4, 6 diamidino-2-phenylindole (DAPI) are obtained from Sigma. Alexa Flour 594 or Alexa Flour 488 is from Invitrogen life Technologies. Collagenase/Dispase is from Roche Biosciences, hyaluronidase type V and Dnase I is from Sigma. α-isonitrosopropiophenone for arginase activity and sulphanilamide for nitrite assay is from Sigma. Bouin's solution is from Sigma. MCSF is from Gibco Life technologies and GMCSF from Peprotech Life sciences. D-luciferin potassium salt is from Caliper Life sciences. CD11b magnetic beads are from Militenyi Biotec.RNA isolation kit from Qiagen. Iscript cDNA synthesis kit and SYBR green are from Biorad (Bio-Rad, Hercules, CA).

Joshi S., Singh A.R., Zulcic M., Bao L., Messer K., Ideker T., Dutkowski J, & Durden D.L. (2014). Rac2 Controls Tumor Growth, Metastasis and M1-M2 Macrophage Differentiation In Vivo. PLoS ONE, 9(4), e95893.

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DOCA-Induced Hypertension Protocol

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Deoxycorticosterone acetate pellets (DOCA, #SM-121) are provided by Innovative Research of America. Isoflurane is the product of Shenzhen Reward Company. Amlodipine besylate tablets (AML, #H10950224) are produced by Pfizer Pharmaceutical Company. Dried rhizomes of Ligusticum chuanxiong Hort (#801003917, chuanxiong) and Gastrodia elata Bl (#801002511, Tianma) were provided by Beijing TongRenTang (Bozhou) Co. Ltd.., which were identified by Professor Hu Benxiang of School of Pharmacy, Shaanxi University of Chinese Medicine, as specified varieties in Pharmacopoeia. p67phox antibody (#sc-374510), p47phox antibody (#sc-17845), Rac1 antibody (#sc-514583), ENaC antibody (#sc-48428), and GAPDH antibody (#sc-47724) were provided by Santa Cruz Biotechnology Company. Lucigenin (#sc-202698A) is produced by Santa Cruz Biotechnology Company. NE enzyme-linked immunosorbent assay kit (ELISA, #BAE-5200) is provided by Rocky Mountain Diagnostics Company. Benzamil (#B2417) and hexamethonium (#H2138) are provided by Sigma-Aldrich.

Zhang Q., Li H., Zhao S., Li F, & Tan Y. (2024). Da-Chuan-Xiong Decoction Ameliorates Sodium Sensitivity and Plasma Norepinephrine via Attenuation of Brain Oxidative Stress in the DOCA-Salt Hypertensive Rats. International Journal of Hypertension, 2024, 2226143.

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