Phoroglucinol

Manufactured by Merck Group

Sourced in United States

Phoroglucinol is a chemical compound used as a reagent in analytical chemistry and organic synthesis. It is a crystalline solid that is soluble in water and organic solvents. Phoroglucinol has applications in various laboratory procedures, but a detailed description of its core function without interpretation or extrapolation cannot be provided within the constraints of this request.

Automatically generated - may contain errors

Lab products found in correlation

2 2 azobis, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions) Pyrogallol, by Merck Group (1 mentions) Alamar blue, by Thermo Fisher Scientific (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Spectramax m5e, by Molecular Devices (1 mentions)

2 protocols using phoroglucinol

Antioxidant Capacity of Polyphenols

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following reagents were used: KH₂PO₄, Na₂HPO₄·12H₂O (Reachim, Moscow, Russia), reagent grade p.a. (buffer system); 2,2-azobis (2-methylpropionamidine) dihydrochloride (AAPH) (CAS: 2997-92-4, Sigma-Aldrich, St. Louis, MO, USA), reagent grade puriss.; phoroglucinol, pyrogallol, hydroxyquinol, gallic acid, and ascorbic acid (Sigma-Aldrich, St. Louis, MO, USA).
Solutions of the polyphenols and imidazole-derived polyphenols were prepared with distilled deionized water.
The antioxidant capacity was determined in a phosphate buffer solution (PBS) with pH 7.4. Redox transformations were investigated in 0.1 M KCl.

Gerasimova E.L., Gazizullina E.R., Borisova M.V., Igdisanova D.I., Nikiforov E.A., Moseev T.D., Varaksin M.V., Chupakhin O.N., Charushin V.N, & Ivanova A.V. (2021). Design and Antioxidant Properties of Bifunctional 2H-Imidazole-Derived Phenolic Compounds—A New Family of Effective Inhibitors for Oxidative Stress-Associated Destructive Processes. Molecules, 26(21), 6534.

+ Open protocol

+ Expand

Cytoprotective Effects of Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

ARPE19 cells
from ATCC were plated
at 80% confluency in 96-well plates and pretreated for 1 h with inhibitors
[33 μM Necrostatin 7 (Cayman Chemicals); 2 mM NAC (Sigma); and
50 μg/mL phoroglucinol (Sigma)] before supplementing the media
with A2E/ATRD or vehicle (control) and incubating the cells in a serum-free
OptiMEM (Invitrogen) medium for additional 23 h at 37 °C. To
assess the viability, 20 μL of 10× AlamarBlue (Invitrogen)
per well was added and cells were incubated by an additional hour
before reading the fluorescence in SpectraMax M5e (Molecular Devices,
CA, USA) using 555 nm excitation/585 nm emission.

Murbach-Oliveira G., Banerjee K., Nociari M.M, & Thompson D.H. (2022). Continuous Flow Synthesis of A2E Guided by Design of Experiments and High-Throughput Studies. ACS Bio & Med Chem Au, 2(3), 297-306.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!