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Ready gel tris hcl gels 4 15

Manufactured by Bio-Rad
Sourced in United States

Ready Gel® Tris-HCl Gels (4–15%) are pre-cast polyacrylamide gels designed for protein separation and electrophoresis. They provide a consistent and reliable separation matrix for analyzing protein samples.

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2 protocols using ready gel tris hcl gels 4 15

1

Gel Electrophoresis Protein Separation

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Ready Gel® Tris-HCl Gels (4–15%) (Bio-Rad, Hercules, CA, USA) were run according to standard protocols 26 (link). The gels were stained with GelCode® Blue Stain Reagent (Thermo Scientific, Waltham, MA, USA) and destained with water.
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2

SDS-PAGE Analysis of rFVIII

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Ready Gel® Tris-HCl Gels (4–15%) (Bio-Rad, USA) were run according to standard protocol [20 (link)]. The rpFVIII sample at 2 µg/well concentration was reduced with 5% β-mercaptoethanol in Bio-Rad Laemmli Sample buffer at 94°C for 5 min, loaded onto gels with Bio-Rad Pre-stained Standards Broad Range and run at 115 V for 2 hours 30 minutes. The gels were stained with GelCode® Blue Stain Reagent (Thermo Scientific, USA) and de-stained in water.
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