Het0016

Manufactured by Merck Group

Sourced in United States

HET0016 is a laboratory equipment product manufactured by Merck Group. It is designed for conducting general laboratory procedures and experiments. The core function of HET0016 is to provide a reliable and consistent platform for various laboratory applications. Details about the specific features and capabilities of this product are not available at this time.

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Lab products found in correlation

P erk sc 7383, by Santa Cruz Biotechnology (1 mentions) Go6976, by Merck Group (1 mentions) Erk 1 sc 93, by Santa Cruz Biotechnology (1 mentions) Cyp4a sc 271983, by Santa Cruz Biotechnology (1 mentions) A1ar antibody, by Merck Group (1 mentions) Pd98059, by Merck Group (1 mentions) Actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Dimethyl sulfoxide (dmso), by Carl Roth (1 mentions) Niflumic acid, by Merck Group (1 mentions) Arachidonic acid, by Cayman Chemical (1 mentions) Sucrose, by Fujifilm (1 mentions) Linoleic acid, by Cayman Chemical (1 mentions) Oleic acid, by Cayman Chemical (1 mentions) Sulfaphenazole, by Cayman Chemical (1 mentions) Stearic acid, by Cayman Chemical (1 mentions) Linolenic acid, by Cayman Chemical (1 mentions) Palmitic acid, by Cayman Chemical (1 mentions) Ketoconazole, by Tokyo Chemical Industry (1 mentions) Lauric acid, by Cayman Chemical (1 mentions) Ticlopidine, by Tokyo Chemical Industry (1 mentions)

4 protocols using het0016

Molecular Signaling Pathway Investigation

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50-(N-ethylcarboxamido)adenosine (NECA), 2-chloro-N cyclopentyladenosine (CCPA), phenylephrine (PE), acetyl choline (ACh), PD98059, and all other chemicals were purchased from Sigma-Aldrich. HET0016 and GO6976 were purchased from Sigma-Aldrich and Cayman chemicals, respectively. Antibodies CYP4A sc-271983, pERK sc-7383, ERK1 sc-93, sEH sc-166961, and actin sc-47778 were purchased from Santa Cruz Biotechnology, Santa Cruz, CA. PKCα was purchased from BD Transduction Labs, San Diego, CA. A₁AR antibody was purchased from Sigma-Aldrich

Yadav V.R., Hong K.L., Zeldin D.C, & Nayeem M.A. (2016). Vascular endothelial over-expression of soluble epoxide hydrolase (Tie2-sEH) enhances adenosine A1 receptor dependent contraction in mouse mesenteric arteries: role of ATP-sensitive K+ channels. Molecular and cellular biochemistry, 422(1-2), 197-206.

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HET0016 treatment in mice

Cited 1 time

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Male mice received an i.p. injection of 10 mg HET0016 per kg of body weight (Sigma‐Aldrich) (Wu et al, 2022 (link)) dissolved in DMSO (Roth) 72 h, 48 h, 24 h, and 30 min before the experiment. Control mice received DMSO.

Karwen T., Kolczynska‐Matysiak K., Gross C., Löffler M.C., Friedrich M., Loza‐Valdes A., Schmitz W., Wit M., Dziaczkowski F., Belykh A., Trujillo‐Viera J., El‐Merahbi R., Deppermann C., Nawaz S., Hastoy B., Demczuk A., Erk M., Wieckowski M.R., Rorsman P., Heinze K.G., Stegner D., Nieswandt B, & Sumara G. (2023). Platelet‐derived lipids promote insulin secretion of pancreatic β cells. EMBO Molecular Medicine, 15(9), e16858.

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Lipid Metabolic Pathway Reagent Procurement

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Tung oil and standard CLA oil were obtained from the Nisshin OilliO Group, Ltd. NADPH, NADP ＋ , NADH, and NAD ＋ were purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan) . Trimethylsilyldiazomethane (TMSN 2 CH 3 ) , trimethylolaminomethane acetate (Tris-actate) , fluvoxamine, ticlopidine, montelukast, fluconazole, chlormethiazole, and ketoconazole were purchased from Tokyo Chemical Industry (Tokyo, Japan) . Indomethacin, niflumic acid, 2-chloroethyl ethyl sulfide (CEES) , 17-octadecynoic acid (17-ODYA) , and HET0016 were purchased from Sigma-Aldrich (St. Louis, MO) . Lauric acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid, prostaglandin A1, sulfaphenazole, and quinidine were obtained from Cayman Chemical Company (Ann Arbor, MI) . All other chemicals (sucrose, EDTA・2Na・2H 2 O, DTT, PMSF) were purchased from Wako Pure Chemical Industries, Ltd.

Wu Q, & Tsuduki T. (2020). CYP4F13 is the Major Enzyme for Conversion of alpha-Eleostearic Acid into cis-9, trans-11-Conjugated Linoleic Acid in Mouse Hepatic Microsomes. Journal of oleo science, 69(9).

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Measuring CYP4A11 Enzyme Activity

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CP, 20-HETE, HET-0016, and clofibrate were obtained from Sigma (St. Louis, MO, USA). Dulbecco's modified eagle medium (DMEM), ECL chemiluminescence kit and SYBR Green Real-time PCR Master Mix were purchased from Toyobo Company (Tokyo, Japan). Trizol kit was purchased from Invitrogen (Carlsbad, CA, USA). CYP4A11 monoclonal antibody and goat anti-mouse IgG were obtained from Santa Cruz (Carlsbad, CA, USA). Streptavidin peroxidase kit, LDH measurement kit, and protease inhibitor cocktail were purchased from Beijing Zhongshan Biotechnology Co. Ltd. (Beijing, China).

Li J., Li D., Tie C., Wu J., Wu Q, & Li Q. (2015). Cisplatin-mediated cytotoxicity through inducing CYP4A 11 expression in human renal tubular epithelial cells. The Journal of toxicological sciences, 40(6).

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