Bs 1004r

Manufactured by Bioss Antibodies

Sourced in United States

The Bs-1004R is a high-performance lab equipment used for various applications in research and analytical settings. It is a compact and versatile instrument designed to perform efficient and reliable tasks. The core function of the Bs-1004R is to enable precise and consistent measurements, making it a valuable tool for researchers and scientists.

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Lab products found in correlation

Ab214462, by Abcam (3 mentions) Progres cf scan, by Jenoptik (2 mentions) Gtx109639, by GeneTex (1 mentions) Biospectrum 810 imaging system, by Analytik Jena (1 mentions) Sc 8414, by Santa Cruz Biotechnology (1 mentions) Immobilon transfer membrane, by Merck Group (1 mentions) Rabbit igg 7074, by Cell Signaling Technology (1 mentions) Gtx100118, by GeneTex (1 mentions) Sc 8008, by Santa Cruz Biotechnology (1 mentions) Ventana benchmark ultra, by Roche (1 mentions) Epr2489, by Abcam (1 mentions) Oc 3f10, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-ACE2 (bs-1004R, (2 citations)

6 protocols using bs 1004r

Immunohistochemical Analysis of ACE2 and TMPRSS2 in Tissues

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Visceral tissues were embedded in paraffin, cut into 3 µm sections and subjected to immunohistochemistry to stain the target. Liver and kidney tissue specimens from mice were stained with ACE2 primary antibody (bs-1004R, Bioss Inc, dilution 50×) or TMPRSS2 primary antibody (ab214462, Abcam, dilution 200×). A Polink-2 Plus HRP DAB Rabbit Bulk kit (D39, GBI LABS) was used for IHC assessment based on the descriptions of the manufacturer, observed with a microscope (Nikon, ECLIPSE, TS100, Japan) and photographed with a photomicrographic camera (Jenoptik, ProgRes CF Scan, CA).

Chien L.H., Deng J.S., Jiang W.P., Chen C.C., Chou Y.N., Lin J.G, & Huang G.J. (2022). Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors. Biomedicine & Pharmacotherapy, 153, 113434.

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Immunohistochemical Analysis of ACE2 and TMPRSS2

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The embedded visceral tissues were sectioned into 3 µm segments, followed by immunohistochemistry to stain the target. ACE2 primary antibody (bs-1004R, Bioss Inc., Woburn, MA, USA, dilution 50×) or TMPRSS2 primary antibody (ab214462, Abcam, dilution 200×) was used to stain liver, kidney, and lung tissue samples from the mice. The IHC assessment was performed utilizing a Polink-2 Plus HRP DAB Rabbit Bulk kit (D39, GBI LABS) in accordance with the guidelines provided by the manufacturer. The observations were conducted using a Nikon microscope (ECLIPSE, TS100, Japan), and images were documented by microscope camera (Jenoptik, ProgRes CF Scan, Fremont, CA, USA).

Chen Y.R., Jiang W.P., Deng J.S., Chou Y.N., Wu Y.B., Liang H.J., Lin J.G, & Huang G.J. (2023). Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro. International Journal of Molecular Sciences, 24(20), 15062.

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Western Blot Analysis of Cellular Proteins

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The cells were dissolved in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40, 1 mM EDTA, and protease inhibitor Cocktail Set III (Calbiochem). Fifty micrograms of protein were fractionated via 10% SDS-PAGE and transferred to Immobilon Transfer Membranes (Millipore). Primary antibodies used in this study included those against β-actin (GTX109639; dilution 1:10,000; GeneTex), poly (ADP-ribose) polymerase (PARP) (9542S; dilution 1:1000; Cell Signaling Technology), ACE2 (bs-1004R; dilution 1:1000; Bioss), p65 (SC-8008; dilution 1:1000; Santa Cruz Biotechnology), p50 (SC-8414; dilution 1:1000; Santa Cruz Biotechnology) and GAPDH (GTX100118; dilution 1:1000; GeneTex). HRP-conjugated secondary antibodies recognizing mouse-IgG (7076) or rabbit-IgG (7074) were purchased from Cell Signaling Technology and used at dilutions of 1:5000–10,000. The chemiluminescence signal was captured on a BioSpectrum 810 Imaging System with VisionWorks software (UVP).

Lee M.C., Chen Y.K., Tsai-Wu J.J., Hsu Y.J, & Lin B.R. (2021). Zinc supplementation augments the suppressive effects of repurposed NF-κB inhibitors on ACE2 expression in human lung cell lines. Life Sciences, 280, 119752.

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Duodenal ACE2 Expression in Celiac Disease

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Ten duodenal samples from CD patients with atrophy and ten without were randomly selected for intestinal detection of the ACE2 receptor. ACE2 immunostaining (bs-1004R, Bioss Antibodies) was performed on endoscopic duodenal biopsies from CD patients using an automated immunostainer (Ventana BenchMark ULTRA, Roche Diagnostics) as previously described [20] (link). ACE2 staining was reported as the percentage of positive enteric cells (0–100%).

Elli L., Facciotti F., Lombardo V., Scricciolo A., Sanders D.S., Vaira V., Barisani D., Vecchi M., Costantino A., Scaramella L., dell'Osso B., Doneda L, & Roncoroni L. (2021). Anti-SARS-CoV-2 immunoglobulin profile in patients with celiac disease living in a high incidence area. Digestive and Liver Disease, 54(1), 3-9.

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Immunohistochemical Analysis of ACE2 and TMPRSS2

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Tissues were preserved in formalin, embedded in a paraffin substrate, sectioned, and incubated with antibodies specific for ACE2 (bs-1004R, Bioss Inc. MA, USA, 1:50) or TMPRSS2 (ab214462, Abcam; MA, USA, 1:200). Sections were observed and photographed under a microscope (Nikon, ECLIPSE, TS100, Tokyo, Japan). Stained areas were quantified using Image J software.

Sun T.K., Huang W.C., Sun Y.W., Deng J.S., Chien L.H., Chou Y.N., Jiang W.P., Lin J.G, & Huang G.J. (2022). Schizophyllum commune Reduces Expression of the SARS-CoV-2 Receptors ACE2 and TMPRSS2. International Journal of Molecular Sciences, 23(23), 14766.

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Flow Cytometric Analysis of Tight Junction Proteins

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The expression of ACE2 in LAD2 was determined by immunostaining with PE-labeled rabbit anti-ACE2 (Bioss, bs-1004R) and detecting with flow cytometry. For detecting tight junction proteins ZO-1, Occludin, Claudin-5 and JAM2 in A549 cells, cells were blocked with 5% BSA in PBS for 1 h at room temperature then incubated with primary antibodies for 2h at 4°C. Primary antibodies against ZO-1 (Invitrogen, 402200), Occludin (Invitrogen, OC-3F10), Claudin-5 (Invitrogen, 4C3C2) and JAM-2 (Abcam, EPR2489), were used. A permeabilizing agent (1%FBS and 0.2% Triton X-100 in PBS) was used for ZO-1 intracellular staining. Cells were washed with FACS buffer and then incubated with Alexa Flour 488-labeled goat anti-rabbit or goat anti-mouse IgG (Invitrogen, A11034; Invitrogen, A11001) for 1 h at 4 °C, then cells were analyzed with flow cytometry.

Wu M., Liu F., Sun J., Li X., He X., Qu Y., Pei R., Sun H., Zhou Y., Yan Q., Wu C., Chen L., Yu G., Chang J., Jin X., Zhao J., Chen X., Zheng Y., & Wang J. (2021). SARS-CoV-2-Triggered Mast Cell Rapid Degranulation Induces Alveolar Epithelial Inflammation and Lung Injury.

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