Arctigenin (Fig. 1 ), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and 4´ ,6-diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). LIVE/DEAD Viability/Cytotoxicity kit was purchased from ThermoFisher Scientific, Inc. (Waltham, MA, USA). PhiPhiLux-G1D2 Caspase-3/7 Assay Kit were purchased from OncoImmunin Inc. (Gaithersburg, MD, USA). Anti-cleaved caspase-3, -8, -9, anti-Fas, anti-PARP, anti-Bcl-2, anti-Bcl-xL, anti-Bax, anti-Bad, anti-Phospho-ERK, anti-total-ERK, anti-phospho-p38, anti-total-p38, anti-phospho-JNK, anti-total-JNK, anti-phospho-NFκB, anti-total-NFκB, anti-phospho-AKT, anti-total-AKT, anti-p53 and anti-β-actin antibodies were supplied by Cell Signaling Technology, Inc. (Danvers, MA, USA).
Anti total nfκb
Manufactured by Cell Signaling Technology
Sourced in United States
Anti total NF-κB is a primary antibody product that recognizes the total form of the NF-κB protein. NF-κB is a transcription factor that plays a central role in regulating the immune response and inflammatory processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti total jnk, by Cell Signaling Technology (3 mentions)
Phospho nf κb, by Cell Signaling Technology (3 mentions)
Anti total p38, by Cell Signaling Technology (2 mentions)
Anti phospho jnk, by Cell Signaling Technology (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
Anti total erk1 2, by Cell Signaling Technology (2 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions)
Anti p53, by Cell Signaling Technology (1 mentions)
Anti fas, by Cell Signaling Technology (1 mentions)
Anti bad, by Cell Signaling Technology (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Anti phospho akt, by Cell Signaling Technology (1 mentions)
Anti total erk, by Cell Signaling Technology (1 mentions)
Anti bcl xl, by Cell Signaling Technology (1 mentions)
Anti total akt, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti phospho nf κb, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole dihydrochloride dapi, by Merck Group (1 mentions)
5 protocols using anti total nfκb
1
Arctigenin-Induced Cell Apoptosis Signaling
2
Protein Extraction and Western Blot Analysis
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Skin samples were pulverized under liquid nitrogen and proteins extracted as previously described (Napoli et al., 2013 (link)). Proteins were then transferred to a nitrocellulose membrane and incubated with the primary anti total NFκB (Cell Signaling Technologies, Danvers, MA), or phospho-NFκB (Cell Signaling Technologies). See Supplemental Information online for details.
3
Protein Expression Analysis in Colon Tissues
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Colon tissues were lysed using a radioimmunoprecipitation assay (RIPA). The protein concentration was detected using the BCA protein assay kit (Life Technologies, Eugene, OR, USA). Thirty milligrams of the proteins were separated by electrophoresis on a 10% SDS polyacrylamide gel and then transferred to PVDF membranes. The samples were incubated with primary antibodies against phospho-ERK1/2 (Cell Signaling Technology, Boston, MA, USA), anti-total ERK1/2 (Cell Signaling Technology), phospho-JNK (Cell Signaling Technology), anti-total JNK (Cell Signaling Technology), phospho-p38 (Cell Signaling Technology), anti-total p38 (Cell Signaling Technology), phospho-NF-κB (Cell Signaling Technology), anti-total NF-κB (Cell Signaling Technology), and HSP90 (Cell Signaling Technology). Proteins were detected using the HRP-conjugated secondary antibody and the chemiluminescent HRP substrate (Millipore, Billerica, MA, USA).
4
Protein Extraction and Western Blot Analysis
5
Amaxa Nucleofection of Cell Lines
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Amaxa® Cell Line Nucleofector® Kit T and Amaxa® Glia Cell Nucleofector® Kit T were purchased from LONZA. Primers for quantitative RT-PCR were synthesized by Life Technologies. SYBR Green for quantitative RT-PCR was purchased from Roche. SP600125, Bay11–7082, SB203580, U0126 and LPS were purchased from Sigma. Amyloid-β 42 (Aβ42) peptide was purchased from AnaSpec. Oligomeric Aβ42 was prepared as previously described (Huang et al., 2015 (link)). Antibodies used in this study are as followed: anti-phospho-p38-MAPK, anti-total-p38-MAPK, anti-phospho-ERK1/2, anti-total-ERK1/2, anti-phospho-JNK, anti-total-JNK, anti-phospho-IκBα, anti-total-IκBα, anti-phosho-NF-κB, anti-total-NF-κB, anti-phospho-c-Jun, anti-total-c-Jun and anti-β-actin were purchased from Cell Signaling Technology; anti-tubulin (Millipore); anti-mouse IgG and anti-rabbit IgG antibody conjugated with horseradish peroxidase (ThermoFisher Scientific).
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