Acetamiprid insecticide

Manufactured by Merck Group

Sourced in France

Acetamiprid is a neonicotinoid insecticide manufactured by Merck Group. It is a white crystalline solid used to control a variety of insect pests. Acetamiprid functions by interfering with the nicotinic acetylcholine receptors in the insect's nervous system, leading to paralysis and death.

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400 mhz spectrometer, by Bruker (3 mentions) Jms 600 mass spectrometer, by JEOL (1 mentions)

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Acetamiprid (29 citations) Insecticides (2 citations)

3 protocols using acetamiprid insecticide

Synthesis and Insecticidal Evaluation of Neonicotinoid Analogs

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Measuring the melting points of all synthesized compounds was performed on a Fisher-John apparatus. By using a Vario EL C, H, N, S analyzer, elemental analyses (C, H, N, and S) were conducted. On a Pye-Unicam SP3-100 spectrophotometer IR spectra were obtained by using the KBr disc technique. ¹H NMR and ¹³C NMR spectra were measured on a Joel 400 MHz and Bruker 400 MHz spectrometers using tetramethylsilane (TMS) as a reference and chemical shifts were reported as ppm. By using a Jeol JMS-600 mass spectrometer, mass spectra were carried out. With thin-layer chromatography, the purity of the title compounds was checked.
Compounds 1–12 were obtained according to the literature procedure [20 ]. Acetamiprid insecticide as a neonicotinoid insecticide was purchased from Sigma-Aldrich. The batches of cowpea aphid, A. craccivora insects were gathered from faba bean, Vicia faba L., fields of Assiut University Experimental Farm during 2017/2018 season. Toxicity of the twelve target compounds plus acetamiprid as reference neonicotinoid insecticide was screened against the collected aphids.

El-Dean A.M., Abd-Ella A.A., Hassanien R., El-Sayed M.E., Zaki R.M, & Abdel-Raheem S.A. (2018). Chemical design and toxicity evaluation of new pyrimidothienotetrahydroisoquinolines as potential insecticidal agents. Toxicology Reports, 6, 100-104.

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Bioactivity Screening of Acetamiprid and Novel Compounds

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Melting points were determined by using a Fisher-Johns apparatus for the compounds 2 and 3. Elemental analysis (C, H, N, and S) was determined by a Vario EL C, H, N, S analyzer. DEPT 135, 1 H NMR and 13 C NMR spectra measurement were accomplished via a Bruker 400 MHz spectrometer in the presence of tetramethylsilane (TMS) as an internal reference. Infrared (IR) spectra were determined by a Pye-Unicam SP3-100 spectrophotometer using the KBr disk technique. Thin-layer chromatography (TLC) was used for the purity check of the synthesized compounds and  (ppm) is the unit of chemical shifts. The field strain of cowpea aphids was collected from faba bean, Vicia faba L., fields of the experimental farm of Assiut University. Compound 1 was prepared according to the reported method 6 and the acetamiprid insecticide was purchased from Sigma-Aldrich (France). acetamiprid insecticide and compounds (2 and 3) were tested against the collected cowpea aphid, Aphis craccivora Koch.

Abdel-Raheem S.A.A., El‐Dean A.M.K., Hassanien R., El-Sayed M.E.A., Sayed M., & Abd-Ella A.A. (2021). Synthesis and spectral characterization of selective pyridine compounds as bioactive agents. Current Chemistry Letters.

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Synthesis and Bioassay of Acetamiprid Analogues

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Melting points were determined by using a Fisher-Johns apparatus for the synthesized compounds. Infrared (IR) spectra were determined by a Pye-Unicam SP3-100 spectrophotometer using the KBr disk technique. Elemental analyses (C, H, N, and S) were determined by a Vario EL C, H, N, S analyzer. DEPT 135, 1 H NMR and 13 C NMR spectra measurements were accomplished via a Bruker 400 MHz spectrometer in the presence of tetramethylsilane (TMS) as an internal reference.  (ppm) is the unit of chemical shifts and thin-layer chromatography (TLC) was used for the purity check of the synthesized compounds. Compound 1 was prepared according to the reported method [6] and the acetamiprid insecticide was purchased from Sigma-Aldrich (France). The field strain of cowpea aphids was gathered from faba bean, Vicia faba L., fields of the experimental farm of Assiut University. Compounds 2 and 3 and acetamiprid were tested against the collected cowpea aphids, A. craccivora.

Abdel-Raheem S.A.A. (2020). Synthesis and biological activity of 2-((3-Cyano-4,6-distyrylpyridin-2-yl) thio) acetamide and its cyclized form. Algerian Journal of Biosciences, 1(2).

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