Bone-marrow-derived dendritic cells were generated from the bone marrow of 8-week-old BALB/c mice from Nanjing Peng Sheng Biological Technology Co. Ltd. according to an established method. For in vitro DC stimulation experiments, we cocultured free R837, PEG-PHEMA-I, or INA nanoparticles [(PEG-PHEMA-I) = 100 μg/ml (phthalocyanine) = 25 μg/ml (R837) = 15 μg/ml] with 106 bone-derived DCs from BALB/c mice for 48 h. After various treatments, DCs stained with anti–CD11c-BB700 (BD Biosciences), anti–CD86-PE-Cy7 (BD Biosciences), and anti–CD80-APC (BD Biosciences) antibodies were then analyzed by flow cytometry (CytoFLEX LX). Data analysis was carried out using FCS express software.
Anti cd11c bb700
Manufactured by BD
Sourced in United States
Anti-CD11c-BB700 is a fluorochrome-conjugated monoclonal antibody that binds to the CD11c cell surface antigen. CD11c is a cell surface receptor that is expressed on dendritic cells, monocytes, and some subsets of lymphocytes. The BB700 fluorochrome allows for detection and analysis of CD11c-positive cells using flow cytometry.
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Lab products found in correlation
Anti cd86 pe cy7, by BD (1 mentions)
Anti cd80 apc, by BD (1 mentions)
Anti cd4 apc, by BD (1 mentions)
Il 12p70, by Thermo Fisher Scientific (1 mentions)
Traut s reagent, by Sangon (1 mentions)
Anti cd80 fitc, by BD (1 mentions)
Cpg odn 1826, by Sangon (1 mentions)
Ova257 264, by Merck Group (1 mentions)
Ova323 339, by Merck Group (1 mentions)
Lyso tracker red, by Solarbio (1 mentions)
Anti cd8a percp cy5, by BD (1 mentions)
Anti cd86 pe, by BD (1 mentions)
Anti cd3e fitc, by BD (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
2 protocols using anti cd11c bb700
1
Bone-Marrow-Derived DC Stimulation
2
Multifunctional Vaccine Delivery System
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Stannous octoate (Sn(Oct)2), polyethyleneimine (PEI), ε-caprolactone, 4-nitrophenyl chloroformate (NPC), N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC), cysteamine hydrochloride, 2,2′-Dithiodipyridine, N-hydroxysuccinimide (NHS) were obtained from Aladdin (China). CpG ODN1826 and traut's reagent were obtained from Sangon Biotech (Shanghai). HOOC-PEG-NH2 were purchased from Ponsure Biological (Shanghai). Carboxyfluorescein succinimidyl ester (CFSE), OVA, OVA257−264, and OVA323−339 were purchased from Sigma-Aldrich (USA). LysoTracker Red and DAPI were purchased from Beijing Solarbio Science & Technology Co.,Ltd. The anti-SIINFEKL/H-2KbPE, anti-CD11c-BB700, anti-CD86-PE, anti-CD80-FITC, anti-CD3e-FITC, anti-CD4-APC and anti-CD8a-PerCP-Cy5.5 were obtained BD Biosciences (USA). IL-12p70, TNF-α and IFN-γ enzyme-linked immunosorbent assay (ELISA) kits were purchased from Invitrogen (USA). FITC—OVA was prepared according to the previous protocol.
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