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Hrp conjugated anti rabbit igg

Manufactured by Vector Laboratories
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HRP-conjugated anti-rabbit IgG is a secondary antibody that binds to rabbit primary antibodies. It is labeled with horseradish peroxidase (HRP), an enzyme that can be used for signal detection in various immunoassays and immunohistochemical applications.

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Lab products found in correlation

Novared, by Vector Laboratories (1 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 anti mouse igg, by Thermo Fisher Scientific (1 mentions) Mouse anti v5, by Thermo Fisher Scientific (1 mentions) Rabbit anti flag, by Merck Group (1 mentions) Mouse anti ha, by Roche (1 mentions) Hrp conjugated anti mouse igg, by Vector Laboratories (1 mentions)

3 protocols using hrp conjugated anti rabbit igg

1

Immunostaining of Astrocytes in WT and RGC-32 KO Mice

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WT and RGC-32 KO mouse astrocytes were plated on Permanox® Chamber Slide culture chambers (Nunc Inc, Rochester, NY) for 24 h, fixed in acetone for 10 min, and then air-dried. The sections were washed in PBS and then incubated with 0.3% hydrogen peroxide diluted in PBS for 10 min to quench the endogenous peroxidase. The slides were incubated with primary rabbit anti-GFAP antibody (Dako Agilent, Santa Clara, CA) overnight at 4°C and then with HRP-conjugated anti-rabbit IgG (Vector Labs) for 30 min. NovaRed (Vector Labs) was used as the chromogenic substrate.
Tatomir A., Beltrand A., Nguyen V., Boodhoo D., Mekala A., Cudrici C., Badea T.C., Muresanu D.F., Rus V, & Rus H. (2021). RGC-32 Regulates Generation of Reactive Astrocytes in Experimental Autoimmune Encephalomyelitis. Frontiers in Immunology, 11, 608294.
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2

Comprehensive Antibody Panel for Studying Chromosome Segregation

Cited 1 time
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Primary antibodies used in in the present study were as follows: anti-XSMC4/XCAP-C (in-house identifier AfR8L, affinity-purified rabbit antibody), anti-XSMC2/XCAP-E (AfR9-6, affinity-purified rabbit antibody), anti-XCAP-D2 (AfR16L, affinity-purified rabbit antibody), anti-XCAP-G (AfR11-3L, affinity-purified rabbit antibody; Hirano and Mitchison, 1994 (link); Hirano et al., 1997 (link)), anti-XCAP-D3 (AfR196-2L, affinity-purified rabbit antibody), anti-XCAP-H2 (AfR201-4, affinity-purified rabbit antibody; Ono et al., 2003 (link)), anti-mSMC4 (AfR326-3L, affinity-purified rabbit antibody; Lee et al., 2011 (link)), anti-XSMC3 (AfR48-5L, affinity-purified rabbit antibody; Losada et al., 1998 (link)), anti–topo IIα (in-house identifier αC1-6, rabbit anti-serum; Hirano and Mitchison, 1993 (link)), anti-histone H3 (CMA301: MABI0301 [RRID AB_1977240]; MBL, mouse antibody), and anti-topo II (AK5: M052-3 [RRID AB_592894]; MBL, mouse antibody). Secondary antibodies used in the present study were as follows: HRP-conjugated anti-rabbit IgG (PI-1000 [RRID AB_2336198], Vector Laboratories, goat antibody), Alexa Fluor 488 anti-rabbit IgG (A-11008 [RRID AB_143165], Thermo Fisher Scientific, goat antibody), Alexa Fluor 568 anti-mouse IgG (A-10037 [RRID AB_2534013], Thermo Fisher Scientific, donkey antibody).
Kinoshita K., Tsubota Y., Tane S., Aizawa Y., Sakata R., Takeuchi K., Shintomi K., Nishiyama T, & Hirano T. (2022). A loop extrusion–independent mechanism contributes to condensin I–mediated chromosome shaping. The Journal of Cell Biology, 221(3), e202109016.
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3

Western Blotting with Antibody Details

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Western blot was carried out following standard procedures using antibodies listed here: mouse anti-V5 (1:5000, Invitrogen), mouse anti-HA (1:2000, Roche), chicken anti-HA (1:2000, Aves), rabbit anti-Flag (1:2000, Sigma-Aldrich), HRP-conjugated anti-mouse IgG (1:5000–1:10000, Vector), HRP-conjugated anti-chicken IgG (1:5000, Jackson Immunoresearch), HRP-conjugated anti-rabbit IgG (1:1000–1:5000, Vector), mouse dCaMKII (Cosmo CAC-TNL-001-CAM 1:1000), rabbit α-p-CaMKIIα (Thr 286) (Santa Cruz sc-12886-R 1:1000), mouse Tubulin DM1A (Abcam ab7291 1:10000).
Ulian-Benitez S., Bishop S., Foldi I., Wentzell J., Okenwa C., Forero M.G., Zhu B., Moreira M., Phizacklea M., McIlroy G., Li G., Gay N.J, & Hidalgo A. (2017). Kek-6: A truncated-Trk-like receptor for Drosophila neurotrophin 2 regulates structural synaptic plasticity. PLoS Genetics, 13(8), e1006968.
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