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Sephadex g 25 prepacked pd10 columns

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Sephadex G-25 prepacked PD10 columns are a type of size-exclusion chromatography media used for the separation and purification of biomolecules. These pre-packed columns provide a convenient and standardized way to perform desalting, buffer exchange, and sample cleanup procedures. The Sephadex G-25 material within the columns is designed to effectively separate small molecules from larger biomolecules, allowing for the rapid and efficient removal of unwanted salts, buffers, or other low molecular weight substances from samples.

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Lab products found in correlation

Ultrapure h2o, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) 1 2 distearoyl sn glycero 3 phosphocholine dspc, by Avanti Polar Lipids (1 mentions) 1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 dspe peg, by Avanti Polar Lipids (1 mentions) Dapi 4 6 diamidino 2 phenylindole, by Thermo Fisher Scientific (1 mentions) Heparin coated tubes, by Thermo Fisher Scientific (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Acetonitrile acn, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Sephadex G-25 PD10 columns G-25 Sephadex columns Sephadex G-25 column Sephadex® G-25 PD10 Sephadex G-25 column Prepacked columns Sephadex columns

4 protocols using sephadex g 25 prepacked pd10 columns

1

Synthesis and Characterization of Polymer-Lipid Nanoparticles

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Materials were purchased from Sigma Aldrich (St Louis, MO) unless otherwise specified. 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG) were purchased from Avanti Polar Lipids (Alabaster, AL). Rhodamine B-labeled dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE-Rhodamine) was acquired from Thermo-Fisher Scientific (Pittsburgh, PA). Diethylaminoethyl methacrylate (DEAEMA) and butyl methacrylate (BMA) were distilled prior to use. 4-Cyano-4-(ethylsulfanylthiocarbonyl) sulfanylpentanoic acid (ECT) and mannose functionalized with ethyl methacrylate (ManEMA) were synthesized and fully characterized as described previously31 (link),32 . Spectra/Por regenerated cellulose dialysis membranes were purchased from Spectrum Laboratories (Houston, TX). Sephadex G-25 prepacked PD10 columns were obtained from GE Healthcare Life Sciences (Pittsburg, PA).
Su F.Y., Chen J., Son H.N., Kelly A.M., Convertine A.J., Ratner D.M, & Stayton P.S. (2018). Polymer-augmented liposomes enhancing antibiotic delivery against intracellular infections. Biomaterials science, 6(7), 1976-1985.
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2

Synthesis and Characterization of GalNAc-MA

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Chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise specified. GalNAc ethyl methacrylate (GalNAc-MA) monomer, 5,6 orthoquinone TQ, and CTQ were purchased from Medchem Source (Federal Way, WA). 4-Cyano-4-(ethylsulfanylthiocarbonyl) sulfanylpentanoic acid (ECT) was purchased from Omm Scientific (Dallas, TX). TQ was purchased from MedKoo Biosciences (Morrisville, NC). Methanol, ultrapure H2O, Dulbecco’s phosphate-buffered saline (DPBS), heparin-coated tubes (BD), DAPI (4′,6-diamidino-2-phenylindole), and Alexa Fluor 488 phalloidin were purchased from Thermo Fisher Scientific (Waltham, MA). Sephadex G-25 prepacked PD10 columns were obtained from GE Healthcare Life Sciences (Pittsburg, PA). High-performance liquid chromatography (HPLC)–grade Methanol and water were purchased from VWR International Ltd. (Radnor, PA).
Pottenger A.E., Roy D., Srinivasan S., Chavas T.E., Vlaskin V., Ho D.K., Livingston V.C., Maktabi M., Lin H., Zhang J., Pybus B., Kudyba K., Roth A., Senter P., Tyson G., Huber H.E., Wesche D., Rochford R., Burke P.A, & Stayton P.S. (2024). Liver-targeted polymeric prodrugs delivered subcutaneously improve tafenoquine therapeutic window for malaria radical cure. Science Advances, 10(16), eadk4492.
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3

Synthesis and Characterization of ECT

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Materials were purchased from Sigma Aldrich (St Louis, MO, USA) unless otherwise specified. 4-Cyano-4-(ethylsulfanylthiocarbonyl) sulfanylpentanoic acid (ECT) was synthesized and fully characterized as described previously [27 ], Spectra/Por regenerated cellulose dialysis membranes were purchased from Spectrum Laboratories (Houston, TX, USA). Sephadex G-25 prepacked PD-10 columns were obtained from GE Healthcare Life Sciences (Pittsburg, PA, USA). Acetonitrile (ACN) and ultrapure H2O were HPLC grade and purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Su F.Y., Srinivasan S., Lee B., Chen J., Convertine A.J., West T.E., Ratner D.M., Skerrett S.J, & Stayton P.S. (2018). Macrophage-Targeted Drugamers With Enzyme-Cleavable Linkers Deliver High Intracellular Drug Dosing and Sustained Drug Pharmacokinetics Against Alveolar Pulmonary Infections. Journal of controlled release : official journal of the Controlled Release Society, 287, 1-11.
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4

Rhodamine B Methacrylate Synthesis and Purification

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Reagents were purchased from Sigma Aldrich unless otherwise specified. Rhodamine B methacrylate was prepared by following the previously published procedure.[67 (link)] Polyethylene glycol methacrylate (PEGMA950) monomer purified using basic aluminum oxide (Brockmann l) before polymerization. Chain transfer agent (CTA), 4-Cyano-4-(phenylcarbonothioylthio)pentanoic acid (CTP) and initiator, 4,4’-Azobis(4-cyanovaleric acid) (ABCVA, V501) were used without further purification. Fasudil hydrochloride was purchased from eNovation Chemicals. Spectra/Por regenerated cellulose dialysis membranes were purchased from Spectrum Laboratories. Sephadex G-25 prepacked PD10 columns were obtained from GE Healthcare Life Sciences.
López C.L., Brempelis K.J., Matthaei J.F., Montgomery K.S., Srinivasan S., Roy D., Huang F., Kreuser S.A., Gardell J.L., Blumenthal I., Chiefari J., Jensen M.C., Crane C.A, & Stayton P.S. (2021). Arming Immune Cell Therapeutics with Polymeric Prodrugs. Advanced healthcare materials, 11(9), e2101944.
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