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Ab63571

Manufactured by Jackson ImmunoResearch
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Ab63571 is a polyclonal antibody produced in goats and purified using affinity chromatography. It is designed to recognize and bind to human GAPDH, a common housekeeping protein. This product can be used in various immunodetection techniques, such as Western blotting, ELISA, and immunohistochemistry, to measure or detect the presence of GAPDH in biological samples.

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Lab products found in correlation

Hrp conjugated goat anti mouse secondary antibody, by Jackson ImmunoResearch (2 mentions) Mab369, by Jackson ImmunoResearch (2 mentions) Goat anti rat hrp conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Hrp conjugated goat anti rabbit secondary antibody, by Jackson ImmunoResearch (1 mentions)

2 protocols using ab63571

1

Cell Surface Biotinylation and Protein Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were cultured in 6-well plates. For cell surface biotinylation assays, cells were labeled with sulfo-NHS-SS-biotin (1.0 mg/mL; Pierce) before purification and analysis via SDS-PAGE/immunoblots. hDAT was detected using a rat monoclonal primary antibody to the N-terminus of hDAT (1:1000) (Millipore Bioscience Research Reagents, catalog number MAB369) and a goat-anti-rat-HRP-conjugated secondary antibody (1:5000) (Jackson ImmunoResearch, catalog number sc-2006). The phosphorylation level of STX1 at Ser14 was detected using a rabbit polyclonal antibody against phospho-S14 either from Castillo et al. (2010) (link) or with a commercially available antibody (1:2500) (Abcam, catalog number ab63571) and with a goat-anti-rabbit-HRP-conjugated secondary antibody (1:5000) (Jackson ImmunoResearch, catalog number sc-3837). Total STX1 was detected using a mouse monoclonal antibody (1:5000) (Sigma, catalog number S 0664) and a goat-anti-mouse-HRP-conjugated secondary antibody (1:5000) (Jackson ImmunoResearch, catalog number sc-2005).
Cartier E., Hamilton P.J., Belovich A.N., Shekar A., Campbell N.G., Saunders C., Andreassen T.F., Gether U., Veenstra-Vanderweele J., Sutcliffe J.S., Ulery-Reynolds P.G., Erreger K., Matthies H.J, & Galli A. (2015). Rare Autism-Associated Variants Implicate Syntaxin 1 (STX1 R26Q) Phosphorylation and the Dopamine Transporter (hDAT R51W) in Dopamine Neurotransmission and Behaviors. EBioMedicine, 2(2), 135-146.
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2

Cell Surface Biotinylation and Syntaxin 1 Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were cultured in 6-well plates. For cell surface biotinylation assays, cells were labeled with sulfo-NHS-SS-biotin (1.0 mg/ml; Pierce) before purification and analysis via SDS-PAGE/immunoblots. hDAT was detected using a rat monoclonal primary antibody to the N-terminus of hDAT (1:1000) (Millipore Bioscience Research Reagents, catalog number MAB369) and a goat-anti-rat-HRP-conjugated secondary antibody (1:5000) (Jackson ImmunoResearch, catalog number sc-2006). The phosphorylation level of STX1 at Ser14 was detected using a rabbit polyclonal antibodies against phospho-S14 either from Castillo et al.39 (link) or with a commercially available antibody (1:2500) (abcam, catalog number ab63571) and with a goat-anti-rabbit-HRP-conjugated secondary antibody (1:5000) (Jackson ImmunoResearch, catalog number sc-3837). Total STX1 was detected using a mouse monoclonal antibody (1:5000) (Sigma, catalog number S 0664) and a goat-anti-mouse-HRP-conjugated secondary antibody (1:5000) (Jackson ImmunoResearch, catalog number sc-2005).
Cartier E., Hamilton P.J., Belovich A.N., Shekar A., Campbell N.G., Saunders C., Andreassen T.F., Gether U., Veenstra-Vanderweele J., Sutcliffe J.S., Ulery-Reynolds P.G., Erreger K., Matthies H.J, & Galli A. (2015). Rare Autism-Associated Variants Implicate Syntaxin 1 (STX1 R26Q) Phosphorylation and the Dopamine Transporter (hDAT R51W) in Dopamine Neurotransmission and Behaviors. EBioMedicine, 2(2), 135-146.
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