205c digital microscope

All specimens were kept in 100% ethanol and examined with an Olympus SZX16 stereomicroscope. Further details were studied under an Olympus BX51 compound microscope. Male and female genitalia were examined and illustrated after dissection. Epigynes were cleaned by Proteinase K solution. Male left palps and female epigynes were imaged with an Olympus BX51 compound microscope in ethanol and in Arabic gum. For SEM, specimens were treated according to Agnarsson (2004) (link). Male palps were cleaned ultrasonically for 1 minute, transferred to 100% ethanol overnight, then air-dried. Palps were glued to round-headed rivets using conductive adhesive, then sputter coated and imaged by a field emission scanning electron microscope (FE-SEM JSM7100F, JEOL, JP). Spider body photos were obtained using a Leica 205C digital microscope. All images were assembled using Helicon Focus image stacking software. All measurements were obtained using a Leica 205C digital microscope and given in millimeters. Eye diameters were taken at the widest point. Leg lengths are given as: total length (femur, patella, tibia, metatarsus, tarsus). All specimens studied are deposited in Centre for Behavioural Ecology and Evolution (CBEE), College of Life Science, Hubei University, Wuhan, China.