Dnase rnase free ddh2o

Genomic DNA was extracted from the 569 goats blood samples according to the instructions of the Animal Blood DNA Extraction Kit (Tiangen, Beijing, China). The DNA was dissolved in enzyme-free sterile water (DNase/RNase-free ddH₂O) (Tiangen, Beijing, China), the DNA sample concentration was measured with a NanoDrop 2000 (Thermo, Waltham, MA, USA), and a 1.2% agarose gel was used to detect DNA quality. The extracted DNA concentrations ranged from 300 to 500 ng/μL and were then diluted to 50 ng/μL for subsequent experiments.
Total RNA extraction was performed according to the instructions of the TRIzol kit (Tiangen, Beijing, China). RNA sample concentration and quality were detected with a NanoDrop 2000 (Thermo, Waltham, MA, USA) and 1.2% agarose gels. Then, the RNA was reverse transcribed into cDNA using a reverse transcription kit (TaKaRa, Kusatsu, Japan) and stored at −20 °C.

All primers, FQ reporter (quenched fluorescent DNA reporter FAM-TTATT-BHQ1) and LF reporter (lateral flow strip reporter, FAM-TTATT-Biotin, FAM-T₂₀-Biotin) were synthesized by Sangon Biotech (Shanghai, China). The detailed sequences are listed in Supplementary Table 1. EnGen^ⓇLbaCas12a and NEBuffer 3.1 (10×) (100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl₂, 100 μg/ml) were purchased from New England Biolabs (MA, USA). The TwistAmp^ⓇBasic kit for RPA and portable real-time fluorometer TwistDX T8 (TwistDX Co. UK) were purchased from TwistDx (Cambridge, United Kingdom). The lateral flow strips (Cat. No. JY0301) and fast nucleic acid release regents (Cat. No. BT0068) were from Tiosbio Biotechnology Co, Ltd. (Beijing, China). Plant DNA extraction kit with magnetic beads (DP342), RNase inhibitor (NG209), and DNase/RNase-free ddH₂O were purchased from Tiangen (Beijing, China). Dithiothreitol (DTT) (Cat. No. 43816) was purchased from Sigma Aldrich (St. Louis, MO, USA). The Qubit^Ⓡ2.0 Fluorometer and the Qubit^ⓇdsDNA HS Assay kit were purchased from Invitrogen (Life technologies, Carlsbad, CA). PDMS prepolymer was purchased from Dow Corning (Midland, USA). The fluorescence quantifications were measured with a Roche LightCycler 480 (Roche, USA).